Microbial biomass and activities associated with subsurface environments contaminated with chlorinated hydrocarbons
- Univ. of Tennessee, Knoxville (USA)
Soil microcosms and enrichment cultures from subsurface sediments and ground waters contaminated with trichloroethylene (TCE) were examined. Total lipids, (1-C{sub 14})acetate incorporation into lipids, and (Me{sup 3}H)thymidine incorporation into DNA were determined in these subsurface environments. In heavily TCE-contaminated zones radioisotopes were not incorporated into lipids or DNA. Radioisotope incorporation occurred in sediments both above and below the TCE plume. Phospholipid fatty acids (PLFA) were not detected, i.e., < 0.5 pmol/L in heavily contaminated groundwater samples. In less contaminated waters, extracted PLFA concentrations were greater than 100 pmol/L and microbial isolates were readily obtained. Degradation of 30-100 mg/L TCE was observed when sediments were amended with a variety of energy sources. Microorganisms in these subsurface sediments have adapted to degrade TCE at concentrations greater than 50 mg/L. 34 refs., 4 figs., 4 tabs.
- OSTI ID:
- 6949467
- Journal Information:
- Geomicrobiology Journal; (USA), Vol. 6:3-4; ISSN 0149-0451
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
CHLORINATED ALIPHATIC HYDROCARBONS
BIODEGRADATION
GROUND WATER
CONTAMINATION
CARBON 14 COMPOUNDS
DNA
ECOLOGICAL CONCENTRATION
LIPIDS
METABOLISM
MICROCOSMS
MICROORGANISMS
SEDIMENTS
SOILS
SUBSURFACE ENVIRONMENTS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AZINES
CHEMICAL REACTIONS
DECOMPOSITION
HALOGENATED ALIPHATIC HYDROCARBONS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
WATER
540320* - Environment
Aquatic- Chemicals Monitoring & Transport- (1990-)
560300 - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques