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Title: Survival and activity of Streptococcus faecalis and escherichia coli in tropical freshwater

The survival of Streptococcus faecalis and Escherichia coli was studied in situ in a tropical rain forest watershed using membrane diffusion chambers. Densities were determined by acridine orange direct count and Coulter Counter. Population activity was determined by microautoradiography, cell respiration, and by nucleic acid composition. Densities of S. faecalis and E. coli decreased less than 1 log unit after 105 hours as measured by direct count methods. Activity as measured by respiration, acridine orange activity, and microautoradiography indicated that both bacteria remained moderately active during the entire study. After 12 hours, E. coli was more active than S. faecalis as measured by nucleic acid composition. In this tropical rain forest watershed, E. coli and S. faecalis survived and remained active for more than 5 days; consequently, both would seem to be unsuitable as indicators of recent fecal contamination in tropical waters.
Authors:
;  [1] ; ;
  1. (Univ. of Puerto Rico, Rio Piedras (Puerto Rico))
Publication Date:
OSTI Identifier:
6942334
Resource Type:
Journal Article
Resource Relation:
Journal Name: Crop Science; (USA); Journal Volume: 18:2
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; PATHOGENS; POPULATION DENSITY; WATER POLLUTION; BIOLOGICAL INDICATORS; AUTORADIOGRAPHY; CONTAMINATION; ESCHERICHIA COLI; FORESTS; FRESH WATER; STREPTOCOCCUS; TROPICAL REGIONS; WATERSHEDS; BACTERIA; HYDROGEN COMPOUNDS; MICROORGANISMS; OXYGEN COMPOUNDS; POLLUTION; WATER 540311* -- Environment, Aquatic-- Basic Studies-- Radiometric Techniques-- (1990-)