Analysis of S-methylmethionine and S-adenosylmethionine in plant tissue by a dansylation, Dual-isotope method

Journal Article · Wed Oct 01 00:00:00 EDT 1986 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(86)90594-4· OSTI ID:6942048

Macnicol, P K

A method is presented for determining the levels of S-methylmethionine (MeMet) and S-adenosylmethionine (AdoMet) in the same plant tissue sample, utilizing readily available equipment. The bottom limit of sensitivity, ca. 100 pmol, can be lowered if required. A trichloracetic acid homogenate of the tissue is supplemented with (carboxyl-/sup 14/C)MeMet and (carboxyl-/sup 14/C)AdoMet. After separation of MeMet and AdoMet from each other and from endogenous homoserine on a phosphocellulose column, the two fractions are heat treated at appropriate pH values to liberate (/sup 14/C)homoserine. Quantitation is via the /sup 3/H//sup 14/C ratio of (/sup 3/H)dansyl-(/sup 14/C)homoserine isolated by thin-layer chromatography. The method is validated with pea cotyledon, corn root, and cauliflower leaf.

• Research Organization: CSIRO, Camberra, Australia
• OSTI ID: 6942048
• Journal Information: Anal. Biochem.; (United States), Vol. 158:1
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
THIN-LAYER CHROMATOGRAPHY
CARBON 14 COMPOUNDS
DUAL-ISOTOPE SUBTRACTION TECHNIQUE
PLANT TISSUES
CHEMICAL ANALYSIS
TRITIUM COMPOUNDS
ADENOSINE
BRASSICA
LEAVES
MAIZE
METHIONINE
ORGANIC SULFUR COMPOUNDS
PISUM
ROOTS
BACTERIA
BODY
CARBOXYLIC ACIDS
CEREALS
CHROMATOGRAPHY
DRUGS
FOOD
GRASS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEGUMINOSAE
LIPOTROPIC FACTORS
MICROORGANISMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
RHIZOBIUM
RIBOSIDES
SEPARATION PROCESSES
TISSUES
TRACER TECHNIQUES
VEGETABLES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550201 - Biochemistry- Tracer Techniques