Endothelin-1 stimulates the release of preloaded ( sup 3 H)D-aspartate from cultured cerebellar granule cells

Journal Article · Fri Mar 16 00:00:00 EST 1990 · Biochemical and Biophysical Research Communications; (USA)

DOI:https://doi.org/10.1016/0006-291X(90)92066-9· OSTI ID:6940531

Lin, W W; Lee, C Y; Chuang, D M ^[1]

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1. NIMH Neuroscience Center, Washington, DC (USA)

We have recently reported that endothelin-1 (ET) induces phosphoinositide hydrolysis in primary cultures of rat cerebellar granule cells. Here we found that ET in a dose-dependent manner (1-30 nM) stimulated the release of preloaded ({sup 3}H)D-aspartate from granule cells. The ET-induced aspartate release was completely blocked in the absence of extracellular Ca{sup 2+}, but was unaffected by 1 mM Co{sup 2+} or 1 microM dihydropyridine derivatives (nisoldipine and nimodipine). At higher concentration (10 microM) of nisoldipine and nimodipine, the release was partially inhibited. Short-term pretreatment of cells with phorbol 12,13-dibutyrate (PDBu) potentiated the ET-induced aspartate release, while long-term pretreatment with PDBu attenuated the release. Long-term exposure of cells to pertussis toxin (PTX), on the other hand, potentiated the ET-induced effects. Our results suggest that ET has a neuromodulatory function in the central nervous system.

• OSTI ID: 6940531
• Journal Information: Biochemical and Biophysical Research Communications; (USA), Vol. 167:2; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ASPARTIC ACID
SECRETION
PEPTIDES
BIOCHEMICAL REACTION KINETICS
PHORBOL ESTERS
BIOLOGICAL EFFECTS
CALCIUM COMPOUNDS
CATIONS
CELL CULTURES
CEREBELLUM
COBALT COMPOUNDS
DOSE-RESPONSE RELATIONSHIPS
ENDOTHELIUM
PORINS
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
BODY
BRAIN
CARBOXYLIC ACIDS
CARCINOGENS
CENTRAL NERVOUS SYSTEM
CHARGED PARTICLES
ESTERS
HYDROGEN COMPOUNDS
IONS
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
REACTION KINETICS
RODENTS
TISSUES
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques