Arginine metabolism in wounds
Arginine metabolism in wounds was investigated in the rat in 1) lambda-carrageenan-wounded skeletal muscle, 2) Schilling chambers, and 3) subcutaneous polyvinyl alcohol sponges. All showed decreased arginine and elevated ornithine contents and high arginase activity. Arginase could be brought to the wound by macrophages, which were found to contain arginase activity. However, arginase was expressed by macrophages only after cell lysis and no arginase was released by viable macrophages in vitro. Thus the extracellular arginase of wounds may derive from dead macrophages within the injured tissue. Wound and peritoneal macrophages exhibited arginase deiminase activity as demonstrated by the conversion of (guanido-/sup 14/C)arginine to radiolabeled citrulline during culture, the inhibition of this reaction by formamidinium acetate, and the lack of prokaryotic contamination of the cultures. These findings and the known metabolic fates of the products of arginase and arginine deiminase in the cellular populations of the wound suggest the possibility of cooperativity among cells for the production of substrates for collagen synthesis.
- Research Organization:
- Rhode Island Hospital, Providence (USA)
- OSTI ID:
- 6939320
- Journal Information:
- Am. J. Physiol.; (United States), Vol. 254
- Country of Publication:
- United States
- Language:
- English
Similar Records
Stimulation by D-glucose of the direct conversion of arginine to citrulline in enterocytes isolated from pig jejunum
Killing of Leishmania parasites in activated murine macrophages is based on an L-arginine-dependent process that produces nitrogen derivatives
Related Subjects
ARGINASE
ARGININE
METABOLISM
WOUNDS
HEALING
AMINO ACIDS
CARBON 14 COMPOUNDS
CITRULLINE
COLLAGEN
ENZYME ACTIVITY
HYDROLASES
MACROPHAGES
MUSCLES
RATS
TRACER TECHNIQUES
UREA
AMIDASES
AMIDES
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
ENZYMES
INJURIES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NON-PEPTIDE C-N HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHAGOCYTES
PROTEINS
RECOVERY
RODENTS
SCLEROPROTEINS
SOMATIC CELLS
VERTEBRATES
550501* - Metabolism- Tracer Techniques
550901 - Pathology- Tracer Techniques