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Title: A preliminary report on zinc-induced resistance to nitrogen mustard toxicity in mice

Previous studies with cultured human normal fibroblasts indicated that treatment of cells with zinc before exposure to alkylating agents enhanced cell survival by seven- to nine-fold. To establish whether a similar zinc-induced protective response could be elicited in vivo, a set of preliminary experiments was carried out in which Balb/cJ mice were treated with zinc chloride (2 mg/kg body weight) or saline by intraperitoneal (ip) injection at 48, 36, 24, and 12 h before ip administration of 4 mg/kg of the alkylating agent nitrogen mustard. Of the animals that received saline before nitrogen mustard, 57% were killed compared with only 20% in the group treated with zinc before administration of the alkylating agent. These results (which almost certainly were achieved with less than optimal induction conditions) provide evidence for the existence, in vivo, of a zinc-inducible process that reduces alkylating agent lethality. 12 refs., 4 figs.
Authors:
;
Publication Date:
OSTI Identifier:
6939034
Report Number(s):
LA-11447
ON: DE89003458
DOE Contract Number:
W-7405-ENG-36
Resource Type:
Technical Report
Resource Relation:
Other Information: Portions of this document are illegible in microfiche products
Research Org:
Los Alamos National Lab., NM (USA)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CELL KILLING; MINIMIZATION; DOSE-RESPONSE RELATIONSHIPS; MODIFICATIONS; NITROGEN MUSTARD; TOXICITY; ZINC CHLORIDES; BIOCHEMICAL REACTION KINETICS; ALKYLATING AGENTS; DISEASE RESISTANCE; FIBROBLASTS; LETHAL DOSES; MICE; RESPONSE MODIFYING FACTORS; AMINES; ANIMAL CELLS; ANIMALS; CHLORIDES; CHLORINE COMPOUNDS; CONNECTIVE TISSUE CELLS; DOSES; HALIDES; HALOGEN COMPOUNDS; KINETICS; MAMMALS; ORGANIC CHLORINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; REACTION KINETICS; RODENTS; SOMATIC CELLS; VERTEBRATES; ZINC COMPOUNDS; ZINC HALIDES 560300* -- Chemicals Metabolism & Toxicology