Uptake of cesium ions by human erythrocytes and perfused rat heart: a cesium-133 NMR study
Cesium-133 NMR studies have been carried out on suspended human erythrocytes and on perfused rat hearts in media containing CsCl. The resulting spectra exhibit two sharp resonances, arising from intra-and extracellular Cs/sup +/, separated in chemical shift by 1.0-1.4 ppm. Thus, intra- and extracellular resonances are easily resolved without the addition of paramagnetic shift reagents required to resolve resonance of the other alkali metal ions. Spin-lattice relaxation times in all cases are monoexponential and significantly shorter (3-4 times) for the intracellular component. When corrections are made for the pulse repetition rate, the total intensity of the intracellular and extracellular Cs/sup +/ resonances in erythrocytes is conserve, implying total observability of the intracellular pool. The uptake of Cs/sup +/ by erythrocytes occurs at approximately one-third the reported rate for K/sup +/ and was reduced by a factor of 2 upon addition of ouabain to the sample. These results indicate that /sup 133/Cs NMR is a promising tool for studying the distribution and transport of cesium ions in biological systems and, in some cases such as uptake by cellular Na,K-ATPase, for analysis of K/sup +/ ion metabolism.
- Research Organization:
- National Institute of Environmental Health Sciences, Research Triangle Park, NC (USA)
- OSTI ID:
- 6938995
- Journal Information:
- Biochemistry; (United States), Vol. 27:10
- Country of Publication:
- United States
- Language:
- English
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CESIUM 133
TISSUE DISTRIBUTION
UPTAKE
ERYTHROCYTES
NUCLEAR MAGNETIC RESONANCE
HEART
CESIUM CHLORIDES
CHEMICAL SHIFT
EXPERIMENTAL DATA
MAN
PERFUSED TISSUES
RATS
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ANIMAL TISSUES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
CESIUM COMPOUNDS
CESIUM ISOTOPES
CHLORIDES
CHLORINE COMPOUNDS
DATA
DISTRIBUTION
HALIDES
HALOGEN COMPOUNDS
INFORMATION
ISOTOPES
MAGNETIC RESONANCE
MAMMALS
MATERIALS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANS
PRIMATES
RESONANCE
RODENTS
STABLE ISOTOPES
TISSUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics