Gibberellin biosynthesis in Gibberlla fujikuroi
- Iowa State Univ., Ames (USA)
Gibberellins (GAs) are a group of plant growth hormones which were first isolated from the fungus Gibberella fujikuori. We have examined the biosynthesis of GAs in this fungus in liquid cultures using HPLC followed by GC-MS. Furthermore we have used cell-free enzyme extracts with {sup 14}C-labeled intermediates to examine the regulation of specific parts of the biosynthetic pathway. GA{sub 3} is the predominant GA in well aerated cultures. GA{sub 4} and GA{sub 7}, intermediates in GA{sub 3} biosynthesis, accumulate in cultures with low levels of dissolved oxygen, but are not detectable in more aerated cultures. Light stimulates GA production in G. fujikuroi cultures grown from young stock. Cell-free enzyme studies indicate that light has no effect on incorporation of mevalonic acid into kaurene, but does significantly stimulate the oxidation of kaurenoic acid.
- OSTI ID:
- 6937684
- Journal Information:
- Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FUNGI
PHYSIOLOGY
GIBBERELLIC ACID
BIOSYNTHESIS
CARBON 14 COMPOUNDS
TRACER TECHNIQUES
CARBOXYLIC ACIDS
ESTERS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LACTONES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
SYNTHESIS
551001* - Physiological Systems- Tracer Techniques