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Title: Interconversion of inositol (1,4,5)-trisphosphate to inositol (1,3,4,5)-tetrakisphosphate and (1,3,4)-trisphosphate in permeabilized adrenal glomerulosa cells is calcium-sensitive and ATP-dependent

Abstract

The metabolism of (/sub 3/H)inositol (1,4,5)-trisphosphate was followed in permeabilized bovine adrenal glomerulosa cells. At low Ca++ concentration (pCa = 7.2), more than 90% of (/sub 3/H)inositol (1,4,5)-trisphosphate had disappeared within 2 min, while two other metabolites, (/sub 3/H)inositol (1,3,4)-trisphosphate and (/sub 3/H)inositol (1,3,4,5)-tetrakisphosphate appeared progressively. At higher Ca++ concentrations (pCa = 5.7 and 4.8), the formation of these two metabolites was markedly increased, but completely abolished if the medium was ATP-depleted. The peak levels for the generation of (/sub 3/H)inositol (1,3,4,5)-tetrakisphosphate (1 min) preceded those of (3H)inositol (1,3,4)-trisphosphate and were closely correlated. These results suggest that, in adrenal glomerulosa cells, the isomer inositol (1,3,4)-trisphosphate is generated from inositol (1,4,5)-trisphosphate via a calcium-sensitive and ATP-dependent phosphorylation/dephosphorylation pathway involving the formation of inositol (1,3,4,5)-tetrakisphosphate.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Univ. Hospital, Geneva, Switzerland
OSTI Identifier:
6937294
Resource Type:
Journal Article
Journal Name:
Biochem. Biophys. Res. Commun.; (United States)
Additional Journal Information:
Journal Volume: 1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; INOSITOLS; METABOLISM; ADRENAL GLANDS; ANIMAL CELLS; CALCIUM; METABOLITES; PHOSPHATES; PHOSPHORYLATION; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ALKALINE EARTH METALS; BODY; CARBOHYDRATES; CHEMICAL REACTIONS; ELEMENTS; ENDOCRINE GLANDS; GLANDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; METALS; MONOSACCHARIDES; ORGANIC COMPOUNDS; ORGANS; OXYGEN COMPOUNDS; PHOSPHORUS COMPOUNDS; SACCHARIDES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Rossier, M F, Dentand, I A, Lew, P D, Capponi, A M, and Vallotton, M B. Interconversion of inositol (1,4,5)-trisphosphate to inositol (1,3,4,5)-tetrakisphosphate and (1,3,4)-trisphosphate in permeabilized adrenal glomerulosa cells is calcium-sensitive and ATP-dependent. United States: N. p., 1986. Web. doi:10.1016/S0006-291X(86)80107-3.
Rossier, M F, Dentand, I A, Lew, P D, Capponi, A M, & Vallotton, M B. Interconversion of inositol (1,4,5)-trisphosphate to inositol (1,3,4,5)-tetrakisphosphate and (1,3,4)-trisphosphate in permeabilized adrenal glomerulosa cells is calcium-sensitive and ATP-dependent. United States. https://doi.org/10.1016/S0006-291X(86)80107-3
Rossier, M F, Dentand, I A, Lew, P D, Capponi, A M, and Vallotton, M B. 1986. "Interconversion of inositol (1,4,5)-trisphosphate to inositol (1,3,4,5)-tetrakisphosphate and (1,3,4)-trisphosphate in permeabilized adrenal glomerulosa cells is calcium-sensitive and ATP-dependent". United States. https://doi.org/10.1016/S0006-291X(86)80107-3.
@article{osti_6937294,
title = {Interconversion of inositol (1,4,5)-trisphosphate to inositol (1,3,4,5)-tetrakisphosphate and (1,3,4)-trisphosphate in permeabilized adrenal glomerulosa cells is calcium-sensitive and ATP-dependent},
author = {Rossier, M F and Dentand, I A and Lew, P D and Capponi, A M and Vallotton, M B},
abstractNote = {The metabolism of (/sub 3/H)inositol (1,4,5)-trisphosphate was followed in permeabilized bovine adrenal glomerulosa cells. At low Ca++ concentration (pCa = 7.2), more than 90% of (/sub 3/H)inositol (1,4,5)-trisphosphate had disappeared within 2 min, while two other metabolites, (/sub 3/H)inositol (1,3,4)-trisphosphate and (/sub 3/H)inositol (1,3,4,5)-tetrakisphosphate appeared progressively. At higher Ca++ concentrations (pCa = 5.7 and 4.8), the formation of these two metabolites was markedly increased, but completely abolished if the medium was ATP-depleted. The peak levels for the generation of (/sub 3/H)inositol (1,3,4,5)-tetrakisphosphate (1 min) preceded those of (3H)inositol (1,3,4)-trisphosphate and were closely correlated. These results suggest that, in adrenal glomerulosa cells, the isomer inositol (1,3,4)-trisphosphate is generated from inositol (1,4,5)-trisphosphate via a calcium-sensitive and ATP-dependent phosphorylation/dephosphorylation pathway involving the formation of inositol (1,3,4,5)-tetrakisphosphate.},
doi = {10.1016/S0006-291X(86)80107-3},
url = {https://www.osti.gov/biblio/6937294}, journal = {Biochem. Biophys. Res. Commun.; (United States)},
number = ,
volume = 1,
place = {United States},
year = {Fri Aug 29 00:00:00 EDT 1986},
month = {Fri Aug 29 00:00:00 EDT 1986}
}