Histamine stimulates chloride secretion in omeprazole-inhibited frog gastric mucosa
Omeprazole (OME) stops hydrogen ion (H) secretion in the histamine (HIST)-stimulated gastric mucosa while the chloride (Cl) which had accompanied the H continues to be pumped into the lumen. This finding suggests that the Cl pump is independent of the H/K ATP-ase driven H pump. To test this hypothesis, 16 Ussing-chambered frog mucosas were exposed to OME prior to HIST stimulation. If the Cl pump is independent, HIST should stimulate Cl secretion in the OME-inhibited mucosa. A 1 hr control (CON) interval preceded exposure to OME (10/sup -4/M) in the nutrient solution. Potential difference (PD), short-circuit current (Isc), resistance (R), H flux (J/sup H/) and Cl flux (J/sup Cl/ with /sup 36/Cl) were measured every 15 min. After 1 hr of OME exposure, HIST (10/sup -5/M) was added to the nutrient solution. The findings demonstrate that HIST stimulates Cl secretion in the OME-inhibited bullfrog gastric mucosa.
- Research Organization:
- Univ. of Utah, Salt Lake City
- OSTI ID:
- 6937073
- Report Number(s):
- CONF-8604222-; TRN: 87-008591
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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CHLORIDES
MEMBRANE TRANSPORT
SECRETION
HISTAMINE
BIOCHEMICAL REACTION KINETICS
ATP-ASE
CHLORINE 36
FROGS
MUCOUS MEMBRANES
TRACER TECHNIQUES
ACID ANHYDRASES
AMINES
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHLORINE COMPOUNDS
CHLORINE ISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
HYDROLASES
IMIDAZOLES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MEMBRANES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHOHYDROLASES
RADIOISOTOPES
REACTION KINETICS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques