Does acetylcholine released within the C1 area of the rostral ventrolateral medulla (RVL) tonically maintain arterial pressure

Arneric, S P; Giuliano, R; Ernsberger, P; Underwood, M D; Reis, D J

Title: Does acetylcholine released within the C1 area of the rostral ventrolateral medulla (RVL) tonically maintain arterial pressure

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Abstract

The RVL, which contains C1 epinephrine neurons (C1 area), plays a major role in the maintenance and reflex control of arterial pressure (AP). Muscarinic cholinergic stimulation of the C1 area is sympathoexcitatory. They sought to determine whether the C1 area of rat: (1) contains choline acetyltransferase (ChAT); (2) releases acetylcholine (ACh); and (3) has ACh receptors. ChAT was immunocytochemically localized to neurons in the C1 area. ChAT activity (pmol/mg prot./40 min; N=5) varied 10-fold over 19 regions microdissected from medulla; it was highest in the hypoglossal and vagal nuclei (203 +/- 63), lowest in the pyramidal tract (19 +/- 4) and moderate in the C1 area (96 +/- 12). Muscarinic binding sites labeled by /sup 3/H-quinuclidinyl benzylate (2 nM) and identified autoradiographically had a similar distribution. Release of /sup 3/H-ACh from (1.0 x 0.5 mm) punches of the C1 area was Ca/sup 2 +/-dependent and graded with respect to the depolarization stimulus (5-55 mM K/sup +/). Bilateral microinjection of atropine sulfate (5.0 nmol/100nl) into the C1 area of urethane anesthetized rats, but not adjacent raphen., lowered MAP (mmHg: - 38 +/- 7; N=7). They conclude that the C1 area contains muscarinic cholinergic receptors and that local neurons synthesize, store andmore »« less

Authors:: Arneric, S P; Giuliano, R; Ernsberger, P; Underwood, M D; Reis, D J

Publication Date:: Wed Mar 05 00:00:00 EST 1986

Research Org.:: Cornell Univ. Medical College, New York, NY

OSTI Identifier:: 6936688

Report Number(s):: CONF-8604222-
Journal ID: CODEN: FEPRA

Resource Type:: Conference

Journal Name:: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

Additional Journal Information:: Journal Volume: 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; ACETYLCHOLINE; RECEPTORS; SECRETION; ARTERIES; BLOOD PRESSURE; AUTORADIOGRAPHY; BIOCHEMICAL REACTION KINETICS; BRAIN; NERVES; TRITIUM COMPOUNDS; AMINES; AMMONIUM COMPOUNDS; AUTONOMIC NERVOUS SYSTEM AGENTS; BLOOD VESSELS; BODY; CARDIOVASCULAR SYSTEM; CENTRAL NERVOUS SYSTEM; DRUGS; ESTERS; KINETICS; LABELLED COMPOUNDS; MEMBRANE PROTEINS; NERVOUS SYSTEM; NEUROREGULATORS; ORGANIC COMPOUNDS; ORGANS; PARASYMPATHOMIMETICS; PROTEINS; QUATERNARY COMPOUNDS; REACTION KINETICS; 550201* - Biochemistry- Tracer Techniques

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                    Arneric, S P, Giuliano, R, Ernsberger, P, Underwood, M D, and Reis, D J. Does acetylcholine released within the C1 area of the rostral ventrolateral medulla (RVL) tonically maintain arterial pressure.  United States: N. p., 1986. 
        Web.

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                    Arneric, S P, Giuliano, R, Ernsberger, P, Underwood, M D, & Reis, D J. Does acetylcholine released within the C1 area of the rostral ventrolateral medulla (RVL) tonically maintain arterial pressure.  United States.

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                    Arneric, S P, Giuliano, R, Ernsberger, P, Underwood, M D, and Reis, D J. 1986.  
        "Does acetylcholine released within the C1 area of the rostral ventrolateral medulla (RVL) tonically maintain arterial pressure".  United States.

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@article{osti_6936688,

  title        = {Does acetylcholine released within the C1 area of the rostral ventrolateral medulla (RVL) tonically maintain arterial pressure},

  author       = {Arneric, S P and Giuliano, R and Ernsberger, P and Underwood, M D and Reis, D J},

  abstractNote = {The RVL, which contains C1 epinephrine neurons (C1 area), plays a major role in the maintenance and reflex control of arterial pressure (AP). Muscarinic cholinergic stimulation of the C1 area is sympathoexcitatory. They sought to determine whether the C1 area of rat: (1) contains choline acetyltransferase (ChAT); (2) releases acetylcholine (ACh); and (3) has ACh receptors. ChAT was immunocytochemically localized to neurons in the C1 area. ChAT activity (pmol/mg prot./40 min; N=5) varied 10-fold over 19 regions microdissected from medulla; it was highest in the hypoglossal and vagal nuclei (203 +/- 63), lowest in the pyramidal tract (19 +/- 4) and moderate in the C1 area (96 +/- 12). Muscarinic binding sites labeled by /sup 3/H-quinuclidinyl benzylate (2 nM) and identified autoradiographically had a similar distribution. Release of /sup 3/H-ACh from (1.0 x 0.5 mm) punches of the C1 area was Ca/sup 2 +/-dependent and graded with respect to the depolarization stimulus (5-55 mM K/sup +/). Bilateral microinjection of atropine sulfate (5.0 nmol/100nl) into the C1 area of urethane anesthetized rats, but not adjacent raphen., lowered MAP (mmHg: - 38 +/- 7; N=7). They conclude that the C1 area contains muscarinic cholinergic receptors and that local neurons synthesize, store and release substantial amounts of ACh. ACh released within the C1 area may participate in the tonic maintenance of resting AP.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/6936688},
  journal      = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number       = ,

  volume       = 45:4,

  place        = {United States},

  year         = {Wed Mar 05 00:00:00 EST 1986},

  month        = {Wed Mar 05 00:00:00 EST 1986}

}

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