Effect of in vitro irradiation and cell cycle-inhibitory drugs on the spontaneous human IgE synthesis in vitro
The in vitro effects of radiation, diterpine forskolin (FK), and hydrocortisone (HC) on the in vitro spontaneous IgE synthesis by peripheral blood B-lymphocytes from atopic patients were investigated. Without affecting cell viability, in vitro irradiation inhibited in a dose-dependent fashion de novo IgE synthesis in vitro by B cells from all patients examined with a mean 40% reduction of in vitro IgE product after treatment with 100 rads. In contrast, the in vitro IgE production by the U266 myeloma cell line was unaffected, even by irradiation with 1600 rads. The addition to B cell cultures from atopic patients of FK consistently resulted in a dose-dependent inhibition of the spontaneous IgE production in vitro. The addition to cultures of 10(-5) and 10(-6) molar concentrations of HC was also usually inhibitory, whereas lower HC concentrations were uneffective or even enhanced the spontaneous in vitro IgE synthesis. When 10(-6) molar concentrations of both HC and FK were combined in culture, a summation inhibitory effect on the spontaneous IgE synthesis was observed. In contrast, neither FK nor HC had inhibitory effect on the in vitro spontaneous IgE synthesis by the U266 myeloma cell line. The spontaneous in vitro IgE synthesis by B cells from patients with Hodgkin's disease, demonstrating high levels of serum IgE, was strongly reduced or virtually abolished after patients underwent total nodal irradiation to prevent the spread of the disease. In addition, the in vitro spontaneous IgE synthesis by B cells from atopic patients was markedly decreased or abolished by in vivo administration of betamethasone.
- Research Organization:
- Univ. of Florence, Italy
- OSTI ID:
- 6936586
- Journal Information:
- J. Allergy Clin. Immunol.; (United States), Vol. 1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
IMMUNOGLOBULINS
BIOSYNTHESIS
LYMPHOCYTES
BIOLOGICAL RADIATION EFFECTS
CELL CYCLE
HYDROCORTISONE
IN VITRO
INHIBITION
ADRENAL HORMONES
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
GLOBULINS
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
KETONES
LEUKOCYTES
MATERIALS
ORGANIC COMPOUNDS
PREGNANES
PROTEINS
RADIATION EFFECTS
SOMATIC CELLS
STEROID HORMONES
STEROIDS
SYNTHESIS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture