(/sup 3/H)leukotriene B/sub 4/ binding to the guinea pig spleen membranes: a rich tissue source for a high affinity leukotriene B/sub 4/ receptor site

Conference · Wed Mar 05 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6936359

Cheng, J B; Kohi, F; Townley, R G

To select a tissue rich for the high affinity leukotriene (LT)B/sub 4/ receptor site, they compared binding of 1 nM (/sup 3/H)LTB/sub 4/ (180 Ci/mmol) to the crude membrane preparations of guinea pig spleen, thymus, lung, uterus, bladder, brain, adrenal gland, small intestine, liver, kidney and heart. They found that the membrane preparations from spleen contained the highest binding activity per mg protein. They characterized the LTB/sub 4/ binding to the spleen preparation in detail. LTB/sub 4/ binding was rapid, reversible, stereoselective and saturable. The data from equilibrium experiments showed a linear Scatchard plot with a K/sub d/ of 1.6 nM and a binding site density of 259 fmol/mg prot. The rank order of agents competing for spleen (/sup 3/H)LTB/sub 4/ binding at 25/sup 0/C was: LTB/sub 4/ (K/sub i/ = 2.8 nM) > 20-OH-LTB/sub 4/ (23 nM) > LTA/sub 4/ (48 nM) > LTA/sub 4/ methyl ester (0.13 ..mu..M) > 20-COOH-LTB/sub 4/ (> 6.6 ..mu..M) greater than or equal to arachidonic acid (0.15 mM) similarly ordered FPL-55,712 (0.11 mM). At 4/sup 0/C, LTB/sub 4/ (2.3 nM) competed at least 10x more effectively than 20-OH-LTB/sub 4/ (29 nM) and 20-COOH-LTB/sub 4/ (> 6.6 ..mu..M). HPLC analysis indicated that incubation of 84 ng LTB/sub 4/ with the spleen membrane at 25/sup 0/C did not result in the formation of 20-OH-LTB/sub 4/ (< 1 ng) in the filtrate. They conclude that guinea pig spleen contains a rich tissue source of high affinity (/sup 3/H)LTB/sub 4/ receptor binding sites.

• Research Organization: Creighton Univ., Omaha, NE
• OSTI ID: 6936359
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ARACHIDONIC ACID
BIOCHEMICAL REACTION KINETICS
RECEPTORS
ADRENAL GLANDS
AFFINITY
BLADDER
BRAIN
CELL MEMBRANES
COMPARATIVE EVALUATIONS
GUINEA PIGS
HEART
KIDNEYS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
LUNGS
SMALL INTESTINE
SPLEEN
THYMUS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UTERUS
ANIMALS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CHROMATOGRAPHY
DIGESTIVE SYSTEM
ENDOCRINE GLANDS
FEMALE GENITALS
GASTROINTESTINAL TRACT
GLANDS
INTESTINES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LYMPHATIC SYSTEM
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
MONOCARBOXYLIC ACIDS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
SEPARATION PROCESSES
URINARY TRACT
VERTEBRATES
550201* - Biochemistry- Tracer Techniques