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Title: ADP-ribosylation of membrane components by pertussis and cholera toxin

Pertussis and cholera toxins are important tools to investigate functional and structural aspects of the stimulatory (N/sub s/) and inhibitory (N/sub i/) regulatory components of adenylyl cyclase. Cholera toxin acts on N/sub s/ by ADP-ribosylating its ..cap alpha../sub s/ subunit; pertussis toxin acts on N/sub i/ by ADP-ribosylating its ..cap alpha..; subunit. By using (/sup 32/P)NAD/sup +/ and determining the transfer of its (/sup 32/P)ADP-ribose moiety to membrane components, it is possible to obtain information on N/sub s/ and N/sub i/. A set of protocols is presented that can be used to study simultaneously and comparatively the susceptibility of N/sub s/ and N/sub i/ to be ADP-ribosylated by cholera and pertussis toxin.
Authors:
; ; ; ; ; ;
Publication Date:
OSTI Identifier:
6936308
Resource Type:
Journal Article
Resource Relation:
Journal Name: Methods Enzymol.; (United States); Journal Volume: 109
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CYCLASES; ENZYME ACTIVITY; TOXINS; BIOCHEMICAL REACTION KINETICS; ADP; CELL MEMBRANES; CHOLERA; EXPERIMENTAL DATA; LABELLED COMPOUNDS; NAD; PHOSPHORUS 32; RIBOSE; TRACER TECHNIQUES; ALDEHYDES; ANTIGENS; BACTERIAL DISEASES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOHYDRATES; CELL CONSTITUENTS; COENZYMES; DATA; DAYS LIVING RADIOISOTOPES; DISEASES; ENZYMES; INFECTIOUS DISEASES; INFORMATION; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LIGHT NUCLEI; LYASES; MATERIALS; MEMBRANES; MONOSACCHARIDES; NUCLEI; NUCLEOTIDES; NUMERICAL DATA; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PENTOSES; PHOSPHORUS ISOTOPES; RADIOISOTOPES; REACTION KINETICS; SACCHARIDES; TOXIC MATERIALS 550201* -- Biochemistry-- Tracer Techniques