Phospholipase A/sub 2/ stimulation during cell secretion in rat basophilic leukemia cells

Journal Article · Wed Jan 01 00:00:00 EST 1986 · J. Immunol.; (United States)

OSTI ID:6935859

Garcia-Gil, M; Siraganian, R P

The bridging of IgE receptors on rat basophilic leukemia cells (RBL-2H3) results in a number of biochemical events that accompany histamine secretion. Prominent among these is the release of arachidonic acid from cellular phospholipids, which could be due to the activation of phospholipase enzymes. In the present experiments they studied the intracellular activation of phospholipase A/sub 2/ (PLA/sub 2/) during histamine release. The enzyme in the homogenates was capable of cleaving arachidonic acid from different phospholipids. The production of lysophospholipids could play a critical role in histamine release from cells. These results demonstrate the activation of PLA/sub 2/ enzyme in cellular homogenates during the secretory process.

• Research Organization: National Institutes of Health, Bethesda, MD
• OSTI ID: 6935859
• Journal Information: J. Immunol.; (United States), Vol. 136:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HISTAMINE
SECRETION
IMMUNOGLOBULINS
RECEPTORS
ARACHIDONIC ACID
CARBON 14
CELL CULTURES
LABELLED COMPOUNDS
LEUKEMIA
LIPASE
PHOSPHOHYDROLASES
PHOSPHOLIPIDS
RADIO-RELEASE ANALYSIS
RATS
ACID ANHYDRASES
AMINES
ANIMALS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON ISOTOPES
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
DISEASES
ENZYMES
ESTERASES
ESTERS
EVEN-EVEN NUCLEI
GLOBULINS
HEMIC DISEASES
HETEROCYCLIC COMPOUNDS
HYDROLASES
IMIDAZOLES
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAMMALS
MEMBRANE PROTEINS
MONOCARBOXYLIC ACIDS
NEOPLASMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOISOTOPES
RODENTS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550301* - Cytology- Tracer Techniques