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Title: The effect of the state of differentiation on labeling of epidermal cell surface glycoproteins

Abstract

Epidermal cells were grown in a medium in which the Ca++ concentration controlled the stage of differentiation. Cell surface molecules of differentiated and undifferentiated cells were compared by lactoperoxidase-catalyzed iodination, by the interaction with /sup 125/I-lectins, and by the metabolic incorporation of L-(/sup 3/H)-fucose. Molecular weights of the labeled components were determined by SDS-PAGE and autoradiography. After lactoperoxidase iodination, most of the radioactivity was found in polypeptide bands of 79,000, 65,000 and 56,000 daltons. The 79,000 band is the most intense for undifferentiated cells but disappears as differentiation proceeds. The 56,000 band is present in normal cells at all stages of differentiation but is absent from neoplastic cells. Glycoproteins reacted with /sup 125/I-lectins were found at 180,000, 130,000 and 85,000 daltons. The 130,000 band was the most prominent for differentiated cells labeled with wheat germ agglutinin but was essentially absent from the undifferentiated cells. With Ricinus communis agglutinin, this band was weaker for undifferentiated than for differentiated cells but was the most intense for both. After metabolic incorporation of tritiated fucose, radioactive glycoproteins were found at 130,000 and 85,000 daltons, with comparable intensities for differentiated and undifferentiated cells.

Authors:
;
Publication Date:
Research Org.:
Department of Dermatology, University of Texas Medical Branch, Galveston
OSTI Identifier:
6933454
Resource Type:
Journal Article
Journal Name:
J. Invest. Dermatol.; (United States)
Additional Journal Information:
Journal Volume: 78:5
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANIMAL CELLS; GROWTH; IODINE 125; ISOTOPE APPLICATIONS; TRITIUM COMPOUNDS; METABOLISM; AGGLUTININS; AUTORADIOGRAPHY; BIOCHEMICAL REACTION KINETICS; CALCIUM IONS; CELL CULTURES; CELL MEMBRANES; CULTURE MEDIA; ENZYME ACTIVITY; EPIDERMIS; GLUCOPROTEINS; IN VITRO; MOLECULAR WEIGHT; QUANTITY RATIO; RATS; TRACER TECHNIQUES; ANIMAL TISSUES; ANIMALS; ANTIBODIES; BETA DECAY RADIOISOTOPES; BODY; CARBOHYDRATES; CELL CONSTITUENTS; CHARGED PARTICLES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; EPITHELIUM; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; IONS; ISOTOPES; KINETICS; LABELLED COMPOUNDS; MAMMALS; MEMBRANES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOISOTOPES; REACTION KINETICS; RODENTS; SACCHARIDES; SKIN; TISSUES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques; 550301 - Cytology- Tracer Techniques

Citation Formats

Brysk, M M, and Snider, J M. The effect of the state of differentiation on labeling of epidermal cell surface glycoproteins. United States: N. p., 1982. Web. doi:10.1111/1523-1747.ep12507473.
Brysk, M M, & Snider, J M. The effect of the state of differentiation on labeling of epidermal cell surface glycoproteins. United States. https://doi.org/10.1111/1523-1747.ep12507473
Brysk, M M, and Snider, J M. 1982. "The effect of the state of differentiation on labeling of epidermal cell surface glycoproteins". United States. https://doi.org/10.1111/1523-1747.ep12507473.
@article{osti_6933454,
title = {The effect of the state of differentiation on labeling of epidermal cell surface glycoproteins},
author = {Brysk, M M and Snider, J M},
abstractNote = {Epidermal cells were grown in a medium in which the Ca++ concentration controlled the stage of differentiation. Cell surface molecules of differentiated and undifferentiated cells were compared by lactoperoxidase-catalyzed iodination, by the interaction with /sup 125/I-lectins, and by the metabolic incorporation of L-(/sup 3/H)-fucose. Molecular weights of the labeled components were determined by SDS-PAGE and autoradiography. After lactoperoxidase iodination, most of the radioactivity was found in polypeptide bands of 79,000, 65,000 and 56,000 daltons. The 79,000 band is the most intense for undifferentiated cells but disappears as differentiation proceeds. The 56,000 band is present in normal cells at all stages of differentiation but is absent from neoplastic cells. Glycoproteins reacted with /sup 125/I-lectins were found at 180,000, 130,000 and 85,000 daltons. The 130,000 band was the most prominent for differentiated cells labeled with wheat germ agglutinin but was essentially absent from the undifferentiated cells. With Ricinus communis agglutinin, this band was weaker for undifferentiated than for differentiated cells but was the most intense for both. After metabolic incorporation of tritiated fucose, radioactive glycoproteins were found at 130,000 and 85,000 daltons, with comparable intensities for differentiated and undifferentiated cells.},
doi = {10.1111/1523-1747.ep12507473},
url = {https://www.osti.gov/biblio/6933454}, journal = {J. Invest. Dermatol.; (United States)},
number = ,
volume = 78:5,
place = {United States},
year = {Sat May 01 00:00:00 EDT 1982},
month = {Sat May 01 00:00:00 EDT 1982}
}