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Title: Detection of TEM-induced reciprocal translocations in F/sub 1/ sons of CD-1 male mice: comparison of sequential fertility evaluation and cytogenetic analysis

Abstract

To determine the positive and negative classification error rates associated with the HTA in our laboratory, F/sub 1/ sons of TEM-exposed CD-1 male mice were evaluated by the sequential fertility method with subsequent cytogenetic analysis. Males who sired three litters of size 10 or less when mated to primiparous females from either the B6C3F/sub 1/ or the BCF/sub 1/ strain were classified as partial steriles. When meiotic chromosomes analyses revealed the presence of at least two cells containing multivalent figures, males were classified as translocation heterozygotes. When the fertility evaluation and the cytogenetic analysis were compared, normal fertility was observed on 5 of 83 (6.02%) translocation-bearing F/sub 1/ males mated to B6C3F/sub 1/ tester females and on 3 of 83 (3.61%) F/sub 1/ males mated to BCF/sub 1/ tester females. Thus, the false-negative error rates were 6.02% and 3.61% with these two tester strains. Multivalent figures were not observed in the meiotic chromosomes of 410 F/sub 1/ males. The false-positive error rates with these two tester strains were 2.93% for the B6C3F/sub 1/ strain and 1.71% for the BCF/sub 1/ strain. The results indicate that nonzero error rates, both false-positive and false-negative, are associated with the sequential mating method HTA.more » In addition, the magnitude of these error rates was influenced not only by the tester female strain but also by the genotype of the F/sub 1/ male.« less

Authors:
; ; ;
Publication Date:
Research Org.:
National Center for Toxicological Research, Jefferson, AR (USA)
OSTI Identifier:
6922027
Resource Type:
Journal Article
Journal Name:
Environ. Mol. Mutagen.; (United States)
Additional Journal Information:
Journal Volume: 11:2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ALKYLATING AGENTS; GENETIC EFFECTS; CHROMOSOMAL ABERRATIONS; CYTOLOGY; FERTILITY; MALES; MEIOSIS; MICE; MUTATION FREQUENCY; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGY; CELL DIVISION; MAMMALS; MUTATIONS; RODENTS; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Morris, S M, Kodell, R L, Domon, O E, and Bishop, J B. Detection of TEM-induced reciprocal translocations in F/sub 1/ sons of CD-1 male mice: comparison of sequential fertility evaluation and cytogenetic analysis. United States: N. p., 1988. Web. doi:10.1002/em.2850110207.
Morris, S M, Kodell, R L, Domon, O E, & Bishop, J B. Detection of TEM-induced reciprocal translocations in F/sub 1/ sons of CD-1 male mice: comparison of sequential fertility evaluation and cytogenetic analysis. United States. https://doi.org/10.1002/em.2850110207
Morris, S M, Kodell, R L, Domon, O E, and Bishop, J B. 1988. "Detection of TEM-induced reciprocal translocations in F/sub 1/ sons of CD-1 male mice: comparison of sequential fertility evaluation and cytogenetic analysis". United States. https://doi.org/10.1002/em.2850110207.
@article{osti_6922027,
title = {Detection of TEM-induced reciprocal translocations in F/sub 1/ sons of CD-1 male mice: comparison of sequential fertility evaluation and cytogenetic analysis},
author = {Morris, S M and Kodell, R L and Domon, O E and Bishop, J B},
abstractNote = {To determine the positive and negative classification error rates associated with the HTA in our laboratory, F/sub 1/ sons of TEM-exposed CD-1 male mice were evaluated by the sequential fertility method with subsequent cytogenetic analysis. Males who sired three litters of size 10 or less when mated to primiparous females from either the B6C3F/sub 1/ or the BCF/sub 1/ strain were classified as partial steriles. When meiotic chromosomes analyses revealed the presence of at least two cells containing multivalent figures, males were classified as translocation heterozygotes. When the fertility evaluation and the cytogenetic analysis were compared, normal fertility was observed on 5 of 83 (6.02%) translocation-bearing F/sub 1/ males mated to B6C3F/sub 1/ tester females and on 3 of 83 (3.61%) F/sub 1/ males mated to BCF/sub 1/ tester females. Thus, the false-negative error rates were 6.02% and 3.61% with these two tester strains. Multivalent figures were not observed in the meiotic chromosomes of 410 F/sub 1/ males. The false-positive error rates with these two tester strains were 2.93% for the B6C3F/sub 1/ strain and 1.71% for the BCF/sub 1/ strain. The results indicate that nonzero error rates, both false-positive and false-negative, are associated with the sequential mating method HTA. In addition, the magnitude of these error rates was influenced not only by the tester female strain but also by the genotype of the F/sub 1/ male.},
doi = {10.1002/em.2850110207},
url = {https://www.osti.gov/biblio/6922027}, journal = {Environ. Mol. Mutagen.; (United States)},
number = ,
volume = 11:2,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1988},
month = {Fri Jan 01 00:00:00 EST 1988}
}