Effect of treatment medium on induction of aneuploidy by nocodazole in Saccharomyces cerevisiae

Journal Article · Fri Jan 01 00:00:00 EST 1988 · Environ. Mol. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2850110305· OSTI ID:6922006

Taylor-Mayer, R E; Mayer, V W; Goin, C J

While studying ways to improve responsiveness of Saccharomyces cerevisiae strain D61.M to agents that induce aneuploidy, the authors noted that nocodazole, which strongly induces aneuploidy when yeast cells are treated in yeast extract-peptone-dextrose (YEPD) medium, had no effect when a synthetic complete (SC) medium was used. Further study revealed that the presence of peptone was necessary for induction. Other aneuploidy-inducing agents, including ethyl acetate, acetone, and methyl benzimidazole-2-yl-carbamate (MBC), were equally active in either medium. Benomyl, which degrades to MBC, was less active in SC than in YEPD medium.

• Research Organization: Slippery Rock Univ., PA (USA)
• OSTI ID: 6922006
• Journal Information: Environ. Mol. Mutagen.; (United States), Vol. 11:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BENZIMIDAZOLES
GENETIC EFFECTS
ANEUPLOIDY
CULTURE MEDIA
PEPTONE
SACCHAROMYCES CEREVISIAE
AZOLES
BIOLOGICAL EFFECTS
FUNGI
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
PLOIDY
PROTEINS
SACCHAROMYCES
YEASTS
560300* - Chemicals Metabolism & Toxicology