Preparative purification of Trichoderma reesei native and core'' Cellobiohydrolase I by electrophoresis and chromatofocussing

Conference · Mon Jan 01 00:00:00 EST 1990

OSTI ID:6921943

Offord, D A; Lee, N E; Woodward, J

The enzymes present in the cellulase complex produced by the fungus Trichoderma reesei have been the subject of considerable attention due to their potential for converting cellulosic materials into glucose for further use in fermentation processes. Cellobiohydrolase I (CBH I) is the major component of crude commercial fungal cellulase preparations and catalyzes the conversion of insoluble cellulose into cellobiose. We have been interested, recently, in the reduction of native and core'' CBH I (the primary structure of CBH I with a catalytic head region) and have needed to develop a method for their preparative purification. We now report that by using electrophoresis and chromatofocussing, preparative quantities of both native and core'' CBH I have been obtained. Since their pI values are different, milligram quantities of core'' CBH I can be generated and purified from the native enzyme by chromatofocussing within 2 hours. 13 refs., 7 figs., 2 tabs.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: DOE/ER
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 6921943
• Report Number(s): CONF-900512-1; ON: DE90010706
• Resource Relation: Conference: 12. symposium on biotechnology for fuels and chemicals, Gatlinburg, TN (USA), 7-11 May 1990
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HYDROLASES
PURIFICATION
CELLOBIOSE
CELLULASE
CELLULOSE
CONVERSION
ELECTROPHORESIS
ENZYMES
FERMENTATION
GELS
GLUCOSE
LIQUID COLUMN CHROMATOGRAPHY
PAPAIN
SYNTHESIS
TRICHODERMA
ALDEHYDES
BIOCONVERSION
CARBOHYDRATES
CHROMATOGRAPHY
COLLOIDS
DISACCHARIDES
DISPERSIONS
EUMYCOTA
FUNGI
GLYCOSYL HYDROLASES
HEXOSES
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PLANTS
POLYSACCHARIDES
SACCHARIDES
SEPARATION PROCESSES
SH-PROTEINASES
550200* - Biochemistry