1,25-dihydroxyvitamin D sub 3 up-regulates the 1,25-dihydroxyvitamin D sub 3 receptor in vivo

Journal Article · Fri Dec 01 00:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.24.9770· OSTI ID:6921575

Strom, M; Sandgren, M E; Brown, T A; DeLuca, H F ^[1]

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1. Univ. of Wisconsin, Madison (USA)

The level of mRNA encoding the 1,25-dihydroxyvitamin D{sub 3} receptor in the intestine of vitamin D-deficient rats given 1,25-dihydroxyvitamin D{sub 3} was determined by Northern blot analysis using a {sup 32}P-labeled cDNA probe to the 1,25-dihydroxyvitamin D{sub 3} receptor. mRNA levels increased 10-fold above deficiency levels at 6 and 12 hr after an intravenous dose of 1,25-dihydroxyvitamin D{sub 3}, returning to predosing levels at 24 hr. Total receptor protein level determined by an immunoradiometric assay was increased 2-fold at 12 hr. No change in unoccupied receptor levels determined by ligand-binding assay was observed during this period. These results suggest that 1,25-dihydroxyvitamin D{sub 3} increases receptor mRNA and total receptor level to maintain constant levels of unoccupied receptor.

• OSTI ID: 6921575
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:24; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHOLECALCIFEROL
BIOLOGICAL EFFECTS
MESSENGER-RNA
AUTORADIOGRAPHY
RECEPTORS
RADIOASSAY
BIOCHEMISTRY
LIGANDS
PHOSPHORUS 32
RECOMBINANT DNA
TRITIUM COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DNA
HYDROGEN COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MEMBRANE PROTEINS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RNA
VITAMIN D
VITAMINS
550201* - Biochemistry- Tracer Techniques