Specific and sensitive radioimmunoassay for 3-methoxy-4-hydroxyphenylethyleneglycol (MOPEG)

Journal Article · Fri Feb 06 00:00:00 EST 1981 · Science; (United States)

DOI:https://doi.org/10.1126/science.7455697· OSTI ID:6921508

Keeton, T K ^[1]; Krutzsch, H; Lovenberg, W

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1. Univ. of Texas Health Science Center, San Antonio

Antibodies that specifically bind the norepinephrine metabolite 3-methoxy-4-hydroxyphenylethyleneglycol (MOPEG) were produced in rabbits after injection of a derivative of MOPEG conjugated with bovine thyroglobulin. A sensitive radioimmunoassay was devised with this antiserum, in which as little as 0.5 nanogram of MOPEG can be accurately measured with a final antibody dilution of 1:180. The antibody appears to be specific for MOPEG, since tritiated MOPEG was not displaced from the antibodies by norepinephrine, epinephrine, dopamine, serotonin, or their major metabolites, including MOPEG-sulfate (333 nanograms each).

• OSTI ID: 6921508
• Journal Information: Science; (United States), Vol. 211
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
ADRENALINE
RADIOIMMUNOASSAY
METABOLITES
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
BRAIN
COMPARATIVE EVALUATIONS
IMMUNE SERUMS
IMMUNITY
METABOLISM
PH VALUE
PLASMA
QUANTITATIVE CHEMICAL ANALYSIS
QUANTITY RATIO
RABBITS
RATS
THYROGLOBULIN
TRITIUM
URINE
ADRENAL HORMONES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
BODY FLUIDS
CENTRAL NERVOUS SYSTEM
CHEMICAL ANALYSIS
DRUGS
GLOBULINS
HORMONES
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MATERIALS
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOASSAY
RADIOISOTOPES
RODENTS
STEROID HORMONES
SYMPATHOMIMETICS
TRACER TECHNIQUES
VERTEBRATES
WASTES
YEARS LIVING RADIOISOTOPES
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550201 - Biochemistry- Tracer Techniques