Tumor necrosis factor (cachetin) decreases adipose cell differentiation in primary cell culture
Cachetin has been shown to effect gene product expression in the established adipose cell line 3T3-L1. Expression of messenger RNA for lipoprotein lipase is suppressed in cultured adipocytes. The purpose of this study was to determine the effect of Cachetin on adipose cell differentiation in primary cell culture. Stromalvascular cells obtained from the inguinal fat pad of 4-5 week old Sprague-Dawley rats were grown in culture for two weeks. During the proliferative growth phase all cells were grown on the same medium and labelled with /sup 3/H-thymidine. Cachetin treatment (10/sup -6/ to 10/sup -10/ M) was initiated on day 5, the initial phase of preadipocyte differentiation. Adipocytes and stromal cells were separated using density gradient, and /sup 3/H-thymidine was determined for both cell types. Thymidine incorporation into adipose cells was decreased maximally (approx. 50%) at 10/sup -10/ M. Stromalvascular cells were not influenced at any of the doses tested. Adipose cell lipid content as indicated by oil red-O staining was decreased by Cachetin. Esterase staining by adipose cells treated with Cachetin was increased indicating an increase in intracellular lipase. These studies show that Cachetin has specific effects on primary adipose cell differentiation.
- Research Organization:
- Univ. of Georgia, Athens
- OSTI ID:
- 6917168
- Report Number(s):
- CONF-8604222-; TRN: 87-005739
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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FAT CELLS
CELL DIFFERENTIATION
PROTEINS
BIOLOGICAL EFFECTS
ADIPOSE TISSUE
CELL CULTURES
MESSENGER-RNA
RATS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AZINES
BODY
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
RNA
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques