Cellulose microfibril assembly and orientation in higher plant cells
Abstract
Freeze-fractured plasma membranes of seedlings of Zea mays L., Burpee's Snowcross, and Pisum sativum L., variety Alsaka, contain terminal complex structures and the impressions of microfibrils from the newest cell wall layer.Terminal complex subunits are on the exoplasmic fracture (EF) face, and rosette subunits are on the protoplasmic fracture (PF) face of the membrane. The association of terminal complexes and rosettes with microfibril tips and their association with newly deposited groups of microfibrils is indirect evidence for their role in microfibril assembly. Microtubules may be responsible for certain orientations of microfibrils, particularly the formation of bands of microfibrils in newly deposited wall layers. However, microfibril orienting mechanisms are more complex, involving factors still present during colchicine treatment. Since UDP-glucose is thought to be a precursor of cellulose microfibrils in higher plant cells, EM radioautography was used to determine the site of incorporation of glucose. However, under the conditions used, glucose was only incorporated from UDP-glucose at the surface of cut or damaged pea stem cells, i.e., in vitro. Thus, incorporation of glucose from UDP-glucose was not useful for probing the patterns of cellulose microfibril synthesis in vivo. 18 references, 8 figures.
- Authors:
-
- Syracuse Univ., NY
- Publication Date:
- OSTI Identifier:
- 6915548
- Report Number(s):
- CONF-8205234-Vol.1
Journal ID: CODEN: JPSSD
- Resource Type:
- Conference
- Journal Name:
- J. Appl. Polym. Sci.: Appl. Polym. Symp.; (United States)
- Additional Journal Information:
- Conference: 9. cellulose conference, Syracuse, NY, USA, 24 May 1982
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; CELL MEMBRANES; MICROSTRUCTURE; MORPHOLOGY; CELL WALL; CELLULOSE; BIOSYNTHESIS; AUTORADIOLYSIS; BIOCHEMICAL REACTION KINETICS; BIOLOGICAL PATHWAYS; COLCHICINE; ELECTRON MICROSCOPY; ORIENTATION; PEAS; PLANT GROWTH; UDPG; ALKALOIDS; ANTIMITOTIC DRUGS; ANTIPYRETICS; AUTOLYSIS; CARBOHYDRATES; CELL CONSTITUENTS; CENTRAL NERVOUS SYSTEM DEPRESSANTS; CHEMICAL RADIATION EFFECTS; CHEMICAL REACTIONS; CHEMISTRY; CRYSTAL STRUCTURE; DECOMPOSITION; DRUGS; FOOD; GLYCOSIDES; GROWTH; KINETICS; MEMBRANES; MICROSCOPY; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; POLYSACCHARIDES; RADIATION CHEMISTRY; RADIATION EFFECTS; RADIOLYSIS; REACTION KINETICS; SACCHARIDES; SYNTHESIS; VEGETABLES; 550800* - Morphology; 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 550300 - Cytology
Citation Formats
Mueller, S C, Maclachlan, G A, and Brown, Jr, R M. Cellulose microfibril assembly and orientation in higher plant cells. United States: N. p., 1983.
Web.
Mueller, S C, Maclachlan, G A, & Brown, Jr, R M. Cellulose microfibril assembly and orientation in higher plant cells. United States.
Mueller, S C, Maclachlan, G A, and Brown, Jr, R M. 1983.
"Cellulose microfibril assembly and orientation in higher plant cells". United States.
@article{osti_6915548,
title = {Cellulose microfibril assembly and orientation in higher plant cells},
author = {Mueller, S C and Maclachlan, G A and Brown, Jr, R M},
abstractNote = {Freeze-fractured plasma membranes of seedlings of Zea mays L., Burpee's Snowcross, and Pisum sativum L., variety Alsaka, contain terminal complex structures and the impressions of microfibrils from the newest cell wall layer.Terminal complex subunits are on the exoplasmic fracture (EF) face, and rosette subunits are on the protoplasmic fracture (PF) face of the membrane. The association of terminal complexes and rosettes with microfibril tips and their association with newly deposited groups of microfibrils is indirect evidence for their role in microfibril assembly. Microtubules may be responsible for certain orientations of microfibrils, particularly the formation of bands of microfibrils in newly deposited wall layers. However, microfibril orienting mechanisms are more complex, involving factors still present during colchicine treatment. Since UDP-glucose is thought to be a precursor of cellulose microfibrils in higher plant cells, EM radioautography was used to determine the site of incorporation of glucose. However, under the conditions used, glucose was only incorporated from UDP-glucose at the surface of cut or damaged pea stem cells, i.e., in vitro. Thus, incorporation of glucose from UDP-glucose was not useful for probing the patterns of cellulose microfibril synthesis in vivo. 18 references, 8 figures.},
doi = {},
url = {https://www.osti.gov/biblio/6915548},
journal = {J. Appl. Polym. Sci.: Appl. Polym. Symp.; (United States)},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 1983},
month = {Sat Jan 01 00:00:00 EST 1983}
}