Accumulation of myoinositol and rubidium ions in galactose-exposed rat lens

Journal Article · Wed Oct 01 00:00:00 EDT 1986 · Invest. Ophthalmol. Visual Sci.; (United States)

OSTI ID:6914167

Kawaba, T; Cheng, H M; Kinoshita, J H

When rat lens is incubated in 30 mM galactose overnight, the extent of accumulation of rubidium ions (Rb) and myoinositol (MI) are affected, as well as the Na-K ATPase activity. Rb accumulation and Na-K ATPase activity are only slightly affected compared to the dramatic drop in MI accumulation. These changes are completely abolished by sorbinil, which blocks polyol formation, or by rendering the galactose medium hypertonic to offset the osmotic effect of polyol formation. On the other hand, the addition of excess MI to the galactose medium had no effect on correcting these changes. The results obtained are consistent with the polyol-osmotic theory of sugar cataract formation.

• Research Organization: Massachusetts Eye and Ear Infirmary, Boston
• OSTI ID: 6914167
• Journal Information: Invest. Ophthalmol. Visual Sci.; (United States), Vol. 10
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CATARACTS
ETIOLOGY
INOSITOL
UPTAKE
RUBIDIUM
ATP-ASE
CRYSTALLINE LENS
ENZYME ACTIVITY
GALACTOSE
IMIDAZOLES
IN VITRO
RATS
ACID ANHYDRASES
ALDEHYDES
ALKALI METALS
ANIMALS
AZOLES
BODY
BODY AREAS
CARBOHYDRATES
DISEASES
ELEMENTS
ENZYMES
EYES
FACE
HEAD
HETEROCYCLIC COMPOUNDS
HEXOSES
HYDROLASES
INOSITOLS
MAMMALS
METALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHOHYDROLASES
RODENTS
SACCHARIDES
SENSE ORGANS
SENSE ORGANS DISEASES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology