skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Cloning and functional expression of a human pancreatic islet glucose-transporter cDNA

Abstract

Previous studies have suggested that pancreatic islet glucose transport is mediated by a high-K{sub m}, low-affinity facilitated transporter similar to that expressed in liver. To determine the relationship between islet and liver glucose transporters, liver-type glucose-transporter cDNA clones were isolated from a human liver cDNA library. The liver-type glucose-transporter cDNA clone hybridized to mRNA transcripts of the same size in human liver and pancreatic islet RNA. A cDNA library was prepared from purified human pancreatic islet tissue and screened with human liver-type glucose-transporter cDNA. The authors isolated two overlapping cDNA clones encompassing 2600 base pairs, which encode a pancreatic islet protein identical in sequence to that of the putative liver-type glucose-transporter protein. Xenopus oocytes injected with synthetic mRNA transcribed from a full-length cDNA construct exhibited increased uptake of 2-deoxyglucose, confirming the functional identity of the clone. These cDNA clones can now be used to study regulation of expression of the gene and to assess the role of inherited defects in this gene as a candidate for inherited susceptibility to non-insulin-dependent diabetes mellitus.

Authors:
; ; ; ; ;  [1]
  1. Washington Univ. School of Medicine, St. Louis, MO (USA)
Publication Date:
OSTI Identifier:
6906544
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America; (USA)
Additional Journal Information:
Journal Volume: 86:22; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GLUCOSE; MEMBRANE TRANSPORT; MEMBRANE PROTEINS; GENE REGULATION; RECOMBINANT DNA; DNA-CLONING; DNA HYBRIDIZATION; ELECTROPHORESIS; LIVER; MAN; MESSENGER-RNA; PANCREAS; PHOSPHORUS 32; TRANSCRIPTION; TRITIUM COMPOUNDS; ALDEHYDES; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CARBOHYDRATES; CLONING; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DNA; ENDOCRINE GLANDS; GLANDS; HEXOSES; HYBRIDIZATION; HYDROGEN COMPOUNDS; ISOTOPES; LIGHT NUCLEI; MAMMALS; MONOSACCHARIDES; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; PRIMATES; PROTEINS; RADIOISOTOPES; RNA; SACCHARIDES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Permutt, M A, Koranyi, L, Keller, K, Lacy, P E, Scharp, D W, and Mueckler, M. Cloning and functional expression of a human pancreatic islet glucose-transporter cDNA. United States: N. p., 1989. Web. doi:10.1073/pnas.86.22.8688.
Permutt, M A, Koranyi, L, Keller, K, Lacy, P E, Scharp, D W, & Mueckler, M. Cloning and functional expression of a human pancreatic islet glucose-transporter cDNA. United States. https://doi.org/10.1073/pnas.86.22.8688
Permutt, M A, Koranyi, L, Keller, K, Lacy, P E, Scharp, D W, and Mueckler, M. 1989. "Cloning and functional expression of a human pancreatic islet glucose-transporter cDNA". United States. https://doi.org/10.1073/pnas.86.22.8688.
@article{osti_6906544,
title = {Cloning and functional expression of a human pancreatic islet glucose-transporter cDNA},
author = {Permutt, M A and Koranyi, L and Keller, K and Lacy, P E and Scharp, D W and Mueckler, M},
abstractNote = {Previous studies have suggested that pancreatic islet glucose transport is mediated by a high-K{sub m}, low-affinity facilitated transporter similar to that expressed in liver. To determine the relationship between islet and liver glucose transporters, liver-type glucose-transporter cDNA clones were isolated from a human liver cDNA library. The liver-type glucose-transporter cDNA clone hybridized to mRNA transcripts of the same size in human liver and pancreatic islet RNA. A cDNA library was prepared from purified human pancreatic islet tissue and screened with human liver-type glucose-transporter cDNA. The authors isolated two overlapping cDNA clones encompassing 2600 base pairs, which encode a pancreatic islet protein identical in sequence to that of the putative liver-type glucose-transporter protein. Xenopus oocytes injected with synthetic mRNA transcribed from a full-length cDNA construct exhibited increased uptake of 2-deoxyglucose, confirming the functional identity of the clone. These cDNA clones can now be used to study regulation of expression of the gene and to assess the role of inherited defects in this gene as a candidate for inherited susceptibility to non-insulin-dependent diabetes mellitus.},
doi = {10.1073/pnas.86.22.8688},
url = {https://www.osti.gov/biblio/6906544}, journal = {Proceedings of the National Academy of Sciences of the United States of America; (USA)},
issn = {0027-8424},
number = ,
volume = 86:22,
place = {United States},
year = {Wed Nov 01 00:00:00 EST 1989},
month = {Wed Nov 01 00:00:00 EST 1989}
}