Cloning and functional expression of a human pancreatic islet glucose-transporter cDNA

Journal Article · Wed Nov 01 00:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.22.8688· OSTI ID:6906544

Permutt, M A; Koranyi, L; Keller, K; Lacy, P E; Scharp, D W; Mueckler, M ^[1]

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1. Washington Univ. School of Medicine, St. Louis, MO (USA)

Previous studies have suggested that pancreatic islet glucose transport is mediated by a high-K{sub m}, low-affinity facilitated transporter similar to that expressed in liver. To determine the relationship between islet and liver glucose transporters, liver-type glucose-transporter cDNA clones were isolated from a human liver cDNA library. The liver-type glucose-transporter cDNA clone hybridized to mRNA transcripts of the same size in human liver and pancreatic islet RNA. A cDNA library was prepared from purified human pancreatic islet tissue and screened with human liver-type glucose-transporter cDNA. The authors isolated two overlapping cDNA clones encompassing 2600 base pairs, which encode a pancreatic islet protein identical in sequence to that of the putative liver-type glucose-transporter protein. Xenopus oocytes injected with synthetic mRNA transcribed from a full-length cDNA construct exhibited increased uptake of 2-deoxyglucose, confirming the functional identity of the clone. These cDNA clones can now be used to study regulation of expression of the gene and to assess the role of inherited defects in this gene as a candidate for inherited susceptibility to non-insulin-dependent diabetes mellitus.

• OSTI ID: 6906544
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:22; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOSE
MEMBRANE TRANSPORT
MEMBRANE PROTEINS
GENE REGULATION
RECOMBINANT DNA
DNA-CLONING
DNA HYBRIDIZATION
ELECTROPHORESIS
LIVER
MAN
MESSENGER-RNA
PANCREAS
PHOSPHORUS 32
TRANSCRIPTION
TRITIUM COMPOUNDS
ALDEHYDES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CLONING
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
ENDOCRINE GLANDS
GLANDS
HEXOSES
HYBRIDIZATION
HYDROGEN COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MONOSACCHARIDES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
SACCHARIDES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques