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Title: Molecular resemblance of an AIDS-associated lymphoma and endemic Burkitt lymphomas: Implications for their pathogenesis

Non-Hodgkin lymphoma is a common feature of AIDS. Approximately 30-40% of these tumors exhibit clinical features suggestive of endemic Burkitt lymphoma: they are aggressive malignancies that occur in association with Epstein-Barr virus infection, they arise in the setting of immunosuppression, and they carry t(8;14) translocations without detectable rearrangement of the MYC oncogene. To understand the molecular basis of these parallels, the authors analyzed a case of Epstein-Barr-positive AIDS-associated undifferentiated lymphoma. Southern blots show that the tumor exhibits immunoglobulin joining segment rearrangement but no rearrangement of the MYC oncogene. Cloning of the rearranged joining segment allowed the isolation of recombinant clones encompassing the translocation breakpoint, and sequencing of the translocation junction disclosed that the breakpoint is situated 7 base pairs from the chromosome 14 site involved in a previously described endemic Burkitt lymphoma translocation. Furthermore, the breakpoint is situated far from MYC on chromosome 8, a constant finding in endemic Burkitt lymphomas. That the molecular architecture of the translocation in this case is strikingly similar to previously analyzed translocations from endemic Burkitt lymphomas strongly suggests that common molecular mechanisms must be operative in the pathogenesis of these tumors.
Authors:
; ;  [1] ;  [2] ;  [3]
  1. (Fels Institute for Cancer Research and Molecular Biology, Philadelphia, PA (USA))
  2. (Univ. of Pennsylvania School of Medicine, Philadelphia (USA))
  3. (Memorial Sloan Kettering Institute, New York, NY (USA))
Publication Date:
OSTI Identifier:
6906459
Resource Type:
Journal Article
Resource Relation:
Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA); Journal Volume: 86:22
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AIDS; PATHOGENESIS; CHROMOSOMAL ABERRATIONS; DNA SEQUENCING; LYMPHOMAS; GENETICS; ENZYME IMMUNOASSAY; HETEROCHROMOSOMES; IMMUNOGLOBULINS; MEN; ONCOGENES; PATIENTS; PHOSPHORUS 32; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOASSAY; BIOLOGY; CHROMOSOMES; DAYS LIVING RADIOISOTOPES; DISEASES; GENES; GLOBULINS; HEMIC DISEASES; IMMUNE SYSTEM DISEASES; IMMUNOASSAY; INFECTIOUS DISEASES; ISOTOPES; LIGHT NUCLEI; MALES; MAMMALS; MAN; MUTATIONS; NEOPLASMS; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; PRIMATES; PROTEINS; RADIOISOTOPES; STRUCTURAL CHEMICAL ANALYSIS; VERTEBRATES; VIRAL DISEASES 550401* -- Genetics-- Tracer Techniques