T-2 mycotoxin inhibits mitochondrial protein synthesis
The authors investigated the effect of T-2 toxin on rat liver mitochondrial protein synthesis. Isolated rat liver mitochondria were supplemented with an S-100 supernatant from rat liver and an external ATP-generating system. An in-vitro assay employing cycloheximide, and inhibitor of cytoplasmic protein synthesis, and chloramphenicol, and inhibitor of mitochondrial protein synthesis, to distinguish mitochondrial protein synthesis from the cytoplasmic process. Amino acid incorporation into mitochondria was dependent on the concentration of mitochondria and was inhibited by chloramphenicol. The rate of uptake of tritium leucine into mitochondrial protein was unaffected by the addition of T-2 toxin and was not a rate-limiting step in incorporation. However, 0.02 micrograms/ml of T-2 toxin decreased the rate of protein synthesis inhibition correlated with the amount of T-2 toxin taken up by the mitochondria. While T-2 toxin is known to inhibit eukaryotic protein synthesis, this is the first time T-2 was shown to inhibit mitochondrial protein synthesis.
- Research Organization:
- Army Medical Research Inst. of Infectious Diseases, Fort Detrick, MD (USA)
- OSTI ID:
- 6905739
- Report Number(s):
- AD-A-192861/3/XAB
- Resource Relation:
- Other Information: Pub. in Toxicon, Vol. 26, No. 1, 77-85(1988)
- Country of Publication:
- United States
- Language:
- English
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BIOLOGICAL WARFARE AGENTS
LEUCINE
UPTAKE
MITOCHONDRIA
PROTEINS
BIOSYNTHESIS
TOXINS
TRITIUM
TRACER TECHNIQUES
AMINO ACIDS
CHLORAMPHENICOL
CYTOPLASM
FUSARIUM
INHIBITION
LIVER
RATS
TOXICITY
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DIGESTIVE SYSTEM
DRUGS
FUNGI
GLANDS
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PARASITES
PLANTS
RADIOISOTOPES
RODENTS
SYNTHESIS
TOXIC MATERIALS
VERTEBRATES
WEAPONS
YEARS LIVING RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques
550900 - Pathology