Cytogenetic effects of cigarette smoke on pulmonary alveolar macrophages of the rat
- Lovelace Biomedical and Environmental Research Institute, Albuquerque, NM (USA)
To determine accurately the potential genetic damage induced by toxic inhaled agents, the cells that receive a high concentration of such agents should be analyzed. Pulmonary alveolar macrophages (PAMs) represent such cells. The authors compared the cytogenetic effects of cigarette smoke on PAMs of rats exposed repeatedly by different methods. This study was part of a larger investigation of the health effects resulting from different methods of exposing rats to cigarette smoke. Fischer 344/N male rats were randomly selected from five different exposure groups. The rats were exposed 6 hr/day, 5 days/week for 22-24 days. All smoke-exposed rats received the same daily concentrations {times} time product of cigarette smoke. Rats were injected intraperitoneally with colchicine at the end of exposure. PAMs were obtained by lung lavage and chromosomal damage was measured. Highly significant smoke-induced differences in both structural and numerical aberrations were observed in continuously exposed rats vs. sham controls, regardless route of exposure. The structural aberrations observed were chromatid-type deletions. Both hypoploid and hyperploid cells were detected. The data suggest that cigarette smoke is clastogenic and may disrupt spindle-fiber formation. These activities may play a role in the induction of human carcinogenesis caused by cigarette smoke exposure.
- DOE Contract Number:
- AC04-76EV01013
- OSTI ID:
- 6905288
- Journal Information:
- Environmental and Molecular Mutagenesis; (USA), Vol. 14:1; ISSN 0893-6692
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MACROPHAGES
CYTOLOGY
TOBACCO SMOKES
GENETIC EFFECTS
CHROMOSOMAL ABERRATIONS
INHALATION
KARYOTYPE
LAVAGE
LUNGS
RATS
AEROSOLS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGY
BODY
COLLOIDS
CONNECTIVE TISSUE CELLS
DISPERSIONS
INTAKE
MAMMALS
MUTATIONS
ORGANS
PHAGOCYTES
RESIDUES
RESPIRATORY SYSTEM
RODENTS
SMOKES
SOLS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology