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Title: Acute ozone-induced lung injury in rats: Structural-functional relationships of developing alveolar edema

As part of a study on the effects of acute ozone stress on the lung surfactant system, we correlated morphometric, biochemical, and functional indices of lung injury using male rats exposed to 3 ppm ozone for 1, 2, 4, and 8 hr. Evaluation of lung mechanics, using the Pulmonary Evaluation and Diagnostic Laboratory System, revealed a significant decrease in dynamic lung compliance (ml/cmH[sub 2]O/kg) from a control value of 0.84 [plus minus] 0.02 (SEM) to 0.72 [plus minus] 0.04 and 0.57 [plus minus] 0.06 at 4 and 8 hr, respectively. At 2 hr there was a transient increase in PaO[sub 2] to 116 torr (control = 92 torr) followed by a decrease at 4 hr (65 torr) and 8 hr (55 torr). Morphometry of lung tissue, fixed by perfusion of fixative via the pulmonary artery at 12 cm H[sub 2]O airway distending pressure, demonstrated an increase in the area of the intravascular compartment at 8 hr, in association with a 65 and 39% replacement of the alveolar area by fluid in ventral and dorsal lung regions, respectively. There was a positive correlation (r = 0.966) between alveolar edema and transudated proteins in lavage fluid. A stepwise multiple regression model, withmore » edema as the dependent variable, suggested that pulmonary vasodilatation, hypoxemia, and depletion of surfactant tubular myelin in lavage fluid were indices for predicting alveolar edema. In a second model, with lavage protein concentration as the dependent variable, decreasing dynamic compliance and hypoxemia were predictors of progressive, intraalveolar transudation of plasma proteins. The above structural-functional relationships support the concept that ozone-induced high-protein alveolar edema is pathogenetically linked to pulmonary hyperemia, deficiency of surfactant tubular myelin, and associated lung dysfunctions.« less
Authors:
; ; ; ; ;  [1]
  1. (James A. Haley Veterans Hospital Research Service, Tampa, FL (United States))
Publication Date:
OSTI Identifier:
6899535
Resource Type:
Journal Article
Resource Relation:
Journal Name: Toxicology and Applied Pharmacology; (United States); Journal Volume: 117:1
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; LUNGS; EDEMA; OZONE; ACUTE EXPOSURE; BIOLOGICAL EFFECTS; RATS; EVALUATION; LABORATORY ANIMALS; LAVAGE; MYELIN; PLASMA; RESPIRATORY SYSTEM DISEASES; ANIMALS; BODY; CELL CONSTITUENTS; CELL MEMBRANES; DISEASES; MAMMALS; MEMBRANES; ORGANS; PATHOLOGICAL CHANGES; RESPIRATORY SYSTEM; RODENTS; SYMPTOMS; VERTEBRATES 560300* -- Chemicals Metabolism & Toxicology; 550200 -- Biochemistry