An investigation of the shedding of macromolecules from the Ehrlich mouse ascites tumor cell
The spontaneous release, or shedding, of cell surface components into the extracellular medium may be important in the determination of several features of the cancer cell phenotype. The release of macromolecules from the Erhlich mouse ascites tumor cell was studied under a variety of experimental conditions to elucidate the origin and the underlying mechanisms of release. The extrinsic macromolecules are a diverse group with apparent molecular weights ranging from 13,500 to 400,000 daltons. External labeling of the cell surface with tritiated 4,4{prime}-diisothiocyano-1,2-diphenylethane-2,2-disulfonic acid (({sup 3}H)H{sub 2}DIDS) reveals a slow loss of labeled components at 4{degrees}C, while at 21{degrees}C and 37{degrees}C an initial rapid loss is followed by a slower release. In vitro metabolic labeling with (1-{sup 14}C)-D-glucosamine hydrochloride, D-(2-{sup 3}H)-mannose and various ({sup 3}H)-L-amino acids results in the appearance of labeled macromolecules in the medium suggesting tumor, not mouse, origin. These data suggest that the extrinsic macromolecules originate from the cell surface. Macromolecules are shed by a temperature and pH sensitive process. These results suggest that a limited proteolytic digestion, or sublethal autolysis, of the cell surface may occur in this system. The macromolecules shed by the Ehrlich cell originate from the surface and are probably released by sublethal autolysis, direct secretion and a passive process.
- Research Organization:
- Texas Univ., San Antonio, TX (USA). Health Science Center
- OSTI ID:
- 6896796
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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