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Title: Stereotaxic implantation of dispersed cell suspensions into brain. A systematic appraisal of cell placement and survival

The application of several recent advances in cell biology, brain implantation, and cell-mediated tumor immunotherapy requires successful and reproducible placement of viable cell suspensions into brain. Stereotaxic implantation is being used to inject cytotoxic lymphocytes into gliomas and to replace dopaminergic cells in parkinsonian models. Systematic assessment of the factors that influence success in implantation of cell suspensions into solid tissues is needed. A model was developed for investigation of stereotaxic implantation using radiolabeled rat lymphokine-activated killer (LAK) cells. Anesthetized rats received microliter injections of cell suspension into the right caudate nucleus. The injection volume, cell concentration, infusion rate, and needle size were varied systematically. The animals were sacrificed 1 hour after injection; the brain was removed and sectioned, and the radioactivity was counted. Three aliquots of the suspension were injected into counting tubes for control analysis. Recovery of radioactivity was expressed as the percent of mean counts per minute (cpm) in the right frontal lobe/mean cpm in the three control tubes. To assess the viability of implanted cells, the right frontal region was mechanically dissociated in media and centrifuged, and the pellet and supernatant were counted. By using small needles and slow infusion of volumes of 10 microliters ormore » less, 85% to 90% of the radioactivity was recovered in the caudate nucleus. At least half of the implanted cells were viable. Consistent, accurate implantation of dispersed cells into brain over a range of volumes, cell concentrations, infusion rates, and needle sizes was achieved.« less
Authors:
; ;
Publication Date:
OSTI Identifier:
6896794
Resource Type:
Journal Article
Resource Relation:
Journal Name: J. Neurosurg.; (United States); Journal Volume: 69:2
Research Org:
National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BRAIN; IMPLANTS; LYMPHOCYTES; SURVIVAL CURVES; BIOLOGICAL MODELS; CELL CULTURES; LYMPHOKINES; RADIOISOTOPES; RATS; SUSPENSIONS; TRACER TECHNIQUES; VIABILITY; ANIMAL CELLS; ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CENTRAL NERVOUS SYSTEM; CONNECTIVE TISSUE CELLS; DISPERSIONS; GROWTH FACTORS; ISOTOPE APPLICATIONS; ISOTOPES; LEUKOCYTES; MAMMALS; MATERIALS; MITOGENS; NERVOUS SYSTEM; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RODENTS; SOMATIC CELLS; VERTEBRATES 550901* -- Pathology-- Tracer Techniques