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Title: Cloning and chromosomal assignment of a human cDNA encoding a T cell- and natural killer cell-specific trypsin-like serine protease

Abstract

A cDNA clone encoding a human T cell- and natural killer cell-specific serine protease was obtained by screening a phage lambdagt10 cDNA library from phytohemagglutinin-stimulated human peripheral blood lymphocytes with the mouse Hanukah factor cDNA clone. In an RNA blot-hybridization analysis, this human Hanukah factor cDNA hybridized with a 1.3-kilobase band in allogeneic-stimulated cytotoxic T cells and the Jurkat cell line, but this transcript was not detectable in normal muscle, liver, tonsil, or thymus. By dot-blot hybridization, this cDNA hybridized with RNA from three cytolytic T-cell clones and three noncytolytic T-cell clones grown in vitro as well as with purified CD16/sup +/ natural killer cells and CD3/sup +/, CD16/sup -/ T-cell large granular lymphocytes from peripheral blood lymphocytes (CD = cluster designation). The nucleotide sequence of this cDNA clone encodes a predicted serine protease of 262 amino acids. The active enzyme is 71% and 77% similar to the mouse sequence at the amino acid and DNA level, respectively. The human and mouse sequences conserve the active site residues of serine proteases--the trypsin-specific Asp-189 and all 10 cysteine residues. The gene for the human Hanukah factor serine protease is located on human chromosome 5. The authors propose that this trypsin-like serinemore » protease may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells.« less

Authors:
; ; ;
Publication Date:
Research Org.:
Stanford Univ. School of Medicine, CA (USA)
OSTI Identifier:
6879595
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 85:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; RECOMBINANT DNA; DNA-CLONING; SERINE PROTEINASES; GENETIC MAPPING; MOLECULAR STRUCTURE; DNA SEQUENCING; HYBRIDIZATION; LIVER; LYMPHOCYTES; MAN; MESSENGER-RNA; MUSCLES; PHOSPHORUS 32; THYMUS; TRYPSIN; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CLONING; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DNA; DNA HYBRIDIZATION; ENZYMES; GLANDS; HYDROLASES; ISOTOPES; LEUKOCYTES; LIGHT NUCLEI; LYMPHATIC SYSTEM; MAMMALS; MAPPING; MATERIALS; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HYDROLASES; PHOSPHORUS ISOTOPES; PRIMATES; RADIOISOTOPES; RNA; SOMATIC CELLS; STRUCTURAL CHEMICAL ANALYSIS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Gershenfeld, H K, Hershberger, R J, Shows, T B, and Weissman, I L. Cloning and chromosomal assignment of a human cDNA encoding a T cell- and natural killer cell-specific trypsin-like serine protease. United States: N. p., 1988. Web. doi:10.1073/pnas.85.4.1184.
Gershenfeld, H K, Hershberger, R J, Shows, T B, & Weissman, I L. Cloning and chromosomal assignment of a human cDNA encoding a T cell- and natural killer cell-specific trypsin-like serine protease. United States. https://doi.org/10.1073/pnas.85.4.1184
Gershenfeld, H K, Hershberger, R J, Shows, T B, and Weissman, I L. 1988. "Cloning and chromosomal assignment of a human cDNA encoding a T cell- and natural killer cell-specific trypsin-like serine protease". United States. https://doi.org/10.1073/pnas.85.4.1184.
@article{osti_6879595,
title = {Cloning and chromosomal assignment of a human cDNA encoding a T cell- and natural killer cell-specific trypsin-like serine protease},
author = {Gershenfeld, H K and Hershberger, R J and Shows, T B and Weissman, I L},
abstractNote = {A cDNA clone encoding a human T cell- and natural killer cell-specific serine protease was obtained by screening a phage lambdagt10 cDNA library from phytohemagglutinin-stimulated human peripheral blood lymphocytes with the mouse Hanukah factor cDNA clone. In an RNA blot-hybridization analysis, this human Hanukah factor cDNA hybridized with a 1.3-kilobase band in allogeneic-stimulated cytotoxic T cells and the Jurkat cell line, but this transcript was not detectable in normal muscle, liver, tonsil, or thymus. By dot-blot hybridization, this cDNA hybridized with RNA from three cytolytic T-cell clones and three noncytolytic T-cell clones grown in vitro as well as with purified CD16/sup +/ natural killer cells and CD3/sup +/, CD16/sup -/ T-cell large granular lymphocytes from peripheral blood lymphocytes (CD = cluster designation). The nucleotide sequence of this cDNA clone encodes a predicted serine protease of 262 amino acids. The active enzyme is 71% and 77% similar to the mouse sequence at the amino acid and DNA level, respectively. The human and mouse sequences conserve the active site residues of serine proteases--the trypsin-specific Asp-189 and all 10 cysteine residues. The gene for the human Hanukah factor serine protease is located on human chromosome 5. The authors propose that this trypsin-like serine protease may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells.},
doi = {10.1073/pnas.85.4.1184},
url = {https://www.osti.gov/biblio/6879595}, journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 85:4,
place = {United States},
year = {Mon Feb 01 00:00:00 EST 1988},
month = {Mon Feb 01 00:00:00 EST 1988}
}