Induction of chromosome damage by ultraviolet light and caffeine: correlation of cytogenetic evaluation and flow karyotype

Journal Article · Mon Mar 01 00:00:00 EST 1982 · Cytometry (Baltimore); (United States)

DOI:https://doi.org/10.1002/cyto.990020504· OSTI ID:6878992

Cremer, C; Cremer, T; Gray, J W

Asynchronously growing cells of a M3-1 Chinese hamster line were ultraviolet (UV) irradiated (lambda . 254 nm) with UV fluences up to 7.5 J/m(2). After irradiation cells were incubated with or without 2 mM caffeine for 20 hr, then mitotic cells were selected by mechanical shaking. Their chromosomes were isolated, stained with Hoechst 33258 and chromomycin A3, and measured flow cytometrically. While the fluorescence distributions of chromosomes (flow karyo-types) from cells treated with UV alone or with caffeine alone were very similar to those of untreated controls, the flow karyo-types of UV + caffeine-treated cells showed a debris continuum that increased with increasing UV fluence suggesting an increased number of chromosome fragments. Visual evaluation of metaphase plates revealed that the percentage of cells with chromosome damage also increased steadily with increasing UV fluence. A high degree of correlation was observed between the relative magnitude of the debris level from flow karyotypes and the percentage of cells with chromosome damage and with generalized chromosomes shattering, respectively, as determined from metaphase spreads.

• Research Organization: Biomedical Sciences Division, Lawrence Livermore National Laboratory, Livermore, CA
• OSTI ID: 6878992
• Journal Information: Cytometry (Baltimore); (United States), Vol. 2:5
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
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CHROMOSOMES
BIOLOGICAL RADIATION EFFECTS
ANIMAL CELLS
CELL CULTURES
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HETEROCYCLIC COMPOUNDS
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ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
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