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Title: Characterization of epidermal growth factor receptors on plasma membranes isolated from rat gastric mucosa

The binding of human epidermal growth factor (hEGF), beta-urogastrone, to plasma membranes isolated from rat gastric mucosa was studied to characterize gastric EGF receptors. The binding of ({sup 125}I)hEGF was temperature dependent, reversible, and saturable. A single class of binding sites for EGF with a dissociation constant of 0.42 nM and maximal binding capacity of 42 fmol/mg protein was suggested. There was little change in the binding of ({sup 125}I)hEGF upon addition of peptide hormones (secretin, insulin), antiulcer drugs (cimetidine), or an ulcer-inducing reagent (aspirin). Cross-linking of ({sup 125}I)hEGF to gastric plasma membranes with the use of disuccinimidyl suberate resulted in the labeling of a protein of 150 kDa. These results indicate the presence of EGF receptors on plasma membranes of rat gastric mucosa.
Authors:
; ; ;  [1]
  1. (Kyoto Univ. Hospital (Japan))
Publication Date:
OSTI Identifier:
6875205
Resource Type:
Journal Article
Resource Relation:
Journal Name: Pharmaceutical Research; (USA); Journal Volume: 7:6
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GROWTH FACTORS; RECEPTORS; CHEMICAL COMPOSITION; ACETYLSALICYLIC ACID; ANTIPYRETICS; BIOCHEMICAL REACTION KINETICS; BIOLOGICAL HALF-LIFE; DOGS; EPIDERMIS; ESCHERICHIA COLI; GASTROINTESTINAL TRACT; IN VITRO; INSULIN; IODINE 125; MAN; MUCOUS MEMBRANES; PROTEINS; RATS; REAGENTS; SECRETIN; TEMPERATURE DEPENDENCE; TRACER TECHNIQUES; ANALGESICS; ANIMAL TISSUES; ANIMALS; BACTERIA; BETA DECAY RADIOISOTOPES; BODY; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM DEPRESSANTS; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; EPITHELIUM; HORMONES; HYDROXY ACIDS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MAMMALS; MEMBRANE PROTEINS; MEMBRANES; MICROORGANISMS; MITOGENS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; PRIMATES; RADIOISOTOPES; REACTION KINETICS; RODENTS; SKIN; TISSUES; VERTEBRATES 550201* -- Biochemistry-- Tracer Techniques