Ketone bodies and islet function: sup 45 Ca handling, insulin synthesis, and release

Journal Article · Sun Jul 01 00:00:00 EDT 1990 · American Journal of Physiology; (USA)

OSTI ID:6875050

Malaisse, W J; Lebrun, P; Yaylali, B; Camara, J; Valverde, I; Sener, A ^[1]

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1. Brussels Free Univ. (Belgium)

D-(-)-beta-Hydroxybutyrate and acetoacetate cause a rapid, sustained, and rapidly reversible stimulation of insulin release from rat pancreatic islets incubated in the presence, but not absence, of D-glucose. This coincides with stimulation of both proinsulin biosynthesis and {sup 45}Ca net uptake. The ketone bodies also decrease {sup 45}Ca outflow from prelabeled islets perifused in the absence of Ca{sup 2+} and, in contrast, enhance effluent radioactivity in the presence of Ca{sup 2+}. In the presence of D-glucose, the secretory response to D-(-)-beta-hydroxybutyrate is concentration related in the 2.5-20 mM range, abolished in the absence of Ca{sup 2+} or presence of KCN, and enhanced by theophylline and forskolin. It corresponds grossly to a shift to the left of the sigmoidal curve relating insulin output to the ambient concentration of D-glucose. The secretory, biosynthetic, and cationic response to acetoacetate is less marked than that evoked by an equimolar concentration of D-(-)-beta-hydroxybutyrate. These features are compatible with the view that the insulinotropic action of ketone bodies would be causally linked to their metabolism in islet cells.

• OSTI ID: 6875050
• Journal Information: American Journal of Physiology; (USA), Vol. 259; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CALCIUM COMPOUNDS
MEMBRANE TRANSPORT
INSULIN
BIOSYNTHESIS
KETONES
BIOLOGICAL FUNCTIONS
ACETOACETATES
BUTYRIC ACID
CALCIUM 45
GLUCOSE
IN VITRO
PANCREAS
PHENYLALANINE
RATS
TRACER TECHNIQUES
ALDEHYDES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CALCIUM ISOTOPES
CARBOHYDRATES
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
FUNCTIONS
GLANDS
HEXOSES
HORMONES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
RADIOISOTOPES
RODENTS
SACCHARIDES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques