The diageotropica mutant of tomato lacks high specific activity auxin sites

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

DOI:https://doi.org/10.1126/science.245.4913.52· OSTI ID:6874314

Hicks, G R; Lomax, T L ^[1]; Rayle, D L ^[2]

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1. Oregon State Univ., Corvallis (USA)
2. San Diego State Univ., CA (USA)

Tomato (Lycopersicum esculentum, Mill) plants homozygous for the single gene diageotropica (dgt) mutation have reduced shoot growth, abnormal vascular tissue, altered leaf morphology, and lack of lateral root branching. These and other morphological and physiological abnormalities suggest that dgt plants are unable to respond to the plant growth hormone auxin (indole-3-acetic acid, IAA). The photoaffinity auxin analogue {sup 3}H-5N{sub 3}-IAA specifically labels a polypeptide doublet of 40 ad 42 kD in membrane preparations from stems of the parental variety VFN8, but not from stems of dgt. In elongation tests, excised dgt roots respond in the same manner to IAA an VFN8 roots. These data suggest that the two polypeptides are part of a physiologically important auxin receptor system which is altered in a tissue-specific manner in the mutant.

• OSTI ID: 6874314
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AUXINS
RECEPTORS
HERBS
GENE MUTATIONS
PLANT STEMS
ELONGATION
BIOCHEMICAL REACTION KINETICS
CELL MEMBRANES
POLYPEPTIDES
TOMATOES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
CELL CONSTITUENTS
FOOD
FRUITS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MEMBRANE PROTEINS
MEMBRANES
MUTATIONS
ORGANIC COMPOUNDS
PEPTIDES
PLANT GROWTH REGULATORS
PLANTS
PROTEINS
REACTION KINETICS
550201* - Biochemistry- Tracer Techniques