Receptors for and effects of insulin and IGF-I in rat glomerular mesangial cells
- Harvard Medical School, Boston, MA (USA) Univ. of Linkoping (Sweden)
Receptors for and biological effects of insulin and insulin-like growth factor I (IGF-I) were studied in cultured rat renal mesangial cells. Specific binding of {sup 125}I-IGF was over 200-fold greater than the specific binding of {sup 125}I-insulin. Fifty percent inhibition of {sup 126}I-insulin binding was obtained with 8 {times} 10{sup {minus}9} M unlabeled insulin. For {sup 125}I-IGF-I, 50% inhibition required 1.8 {times} 10{sup {minus}9} M unlabeled IGF-I. {sup 125}I-IGF-I was also displaced by IGF-II and insulin but at 10- and 100-fold lower potencies, respectively, than IGF-I. Cross-linking of {sup 125}I-insulin and {sup 125}I-IGF-I to their receptors, using disuccinimidyl suberate (DSS), and identification of the receptor with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography showed a band with a molecular mass of 135 kDa, probably corresponding to the {alpha}-subunit of the insulin receptor and a major band with a molecular mass of 145 kDa for the {alpha}-subunit of the IGF-I receptor. Both insulin and IGF-I stimulated the incorporation of ({sup 3}H)thymidine into DNA. A half-maximal effect was obtained at 1.6 {times} 10{sup {minus}8} M for insulin and 1.2 {times} 10{sup {minus}9} M for IGF-I. No additive effect on DNA synthesis was observed. Insulin at 8 {times} 10{sup {minus}10} M increased the accumulation of ({sup 14}C)glucose in mesangial cells, whereas IGF-I was 10-fold less potent.
- OSTI ID:
- 6871410
- Journal Information:
- American Journal of Physiology; (USA), Vol. 254:3; ISSN 0002-9513
- Country of Publication:
- United States
- Language:
- English
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AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
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DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
ELECTROPHORESIS
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HETEROCYCLIC COMPOUNDS
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INTERMEDIATE MASS NUCLEI
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ISOTOPE APPLICATIONS
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KIDNEYS
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550201* - Biochemistry- Tracer Techniques