Effect of lipopolysaccharide on protein accumulation by murine peritoneal macrophages: the correlation to activation for macrophage tumoricidal function
The protein synthetic patterns of tumoricidal murine peritoneal macrophage populations have been compared to those of non-tumoricidal populations utilizing two dimensional polyacrylamide gel electrophoresis (2D PAGE) of (/sup 35/S)-methionine-labeled proteins. While the protein synthetic patterns exhibited by resident, inflammatory and activated macrophages had numerous common features which distinguished them from the other normal non-macrophage cell types examined, unique proteins also distinguished each macrophage population from the others. Peritoneal macrophages elicited by treatment with heat killed Propionibacterium acnes, the live, attenuated Mycobacterium bovis strain BCG, Listeria monocytogenes and the protozoan flagellate Trypanosoma rhodesiense, all exhibited tumoricidal activity in 16h or 72h functional assays, and shared a common protein synthetic profile which differentiated them from the synthetic patterns characteristic of the non-tumoricidal resident and inflammatory macrophages.
- Research Organization:
- Temple Univ., Philadelphia, PA (USA)
- OSTI ID:
- 6868522
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LIPOPOLYSACCHARIDES
BIOCHEMICAL REACTION KINETICS
MACROPHAGES
BIOLOGICAL FUNCTIONS
PROTEINS
BIOLOGICAL ACCUMULATION
TUMOR CELLS
INHIBITION
BACTERIA
ELECTROPHORESIS
METHIONINE
MICE
MYCOBACTERIUM
PERITONEUM
PROTOZOA
SULFUR 35
TRACER TECHNIQUES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
FUNCTIONS
INVERTEBRATES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LIPIDS
LIPOTROPIC FACTORS
MAMMALS
MEMBRANES
MICROORGANISMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHAGOCYTES
POLYSACCHARIDES
RADIOISOTOPES
REACTION KINETICS
RODENTS
SACCHARIDES
SEROUS MEMBRANES
SOMATIC CELLS
SULFUR ISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques