Inheritance of a functional mouse metallothionein gene in tobacco

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6867779

Maiti, I B; Wagner, G J; Yeargan, R; Hunt, A G ^[1]

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1. Univ. of Kentucky, Lexington (USA)

Morphologically normal plants were obtained from progeny (Ro, R1 and R2) originating from tobacco leaf tissue transformed with Agrobacterium tumefaciens containing a chimeric gene for kanamycin resistance an the mouse metallothionein cDNA gene directed by the constitutive promote 35S from CaMV. Integration and expression in R1 progeny was demonstrated by Southern, Northern blot analysis and metallothionein assay. Kanamycin resistance analysis of R1 and R2 progeny showed inheritance to be as a dominant Mendelian trait. Seedlings obtained from self-fertilized transgenic tobacco are more tolerant to cadmium stress than nontransformed controls. Cadmium accumulation in leaves of transgenic seedlings exposed to a low, field-like Cd concentration was about 20% lower than that in nontransformed controls.

• OSTI ID: 6867779
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
14 SOLAR ENERGY
CADMIUM
TOXICITY
METALLOTHIONEIN
GENES
NICOTIANA
GENE REGULATION
DOMINANT MUTATIONS
GENETIC ENGINEERING
MICE
TOBACCO
ANIMALS
ELEMENTS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MAMMALS
METALLOPROTEINS
METALS
MUTATIONS
ORGANIC COMPOUNDS
PLANTS
PROTEINS
RODENTS
VERTEBRATES
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