Study of cell degranulation with simultaneous microscope imaging and capillary electrophoresis
Abstract
The physical release of single granules from individual rat peritoneal mast cells (RPMCs) was monitored by video recording of the degranulating cells by a high-resolution charge-coupled device (CCD) microscope system. The bright granular core disappears from the image as the vesicular content is dissolved on contact with the extra-cellular fluid. After a fixed time delay, the exocytotic product, serotonin, was detected by capillary electrophoresis coupled with laser-induced native fluorescence (LINF-CE). The timing of the two events is mostly correlated, which supports a fast release mechanism of the granular products. {copyright} {ital 1999} {ital Society for Applied Spectroscopy}
- Authors:
-
- Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011 (United States)
- Publication Date:
- Research Org.:
- Ames National Laboratory
- OSTI Identifier:
- 686512
- DOE Contract Number:
- W-7405-ENG-82
- Resource Type:
- Journal Article
- Journal Name:
- Applied Spectroscopy
- Additional Journal Information:
- Journal Volume: 53; Journal Issue: 7; Other Information: PBD: Jul 1999
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; OPTICAL MICROSCOPY; PROTEINS; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; CHEMICAL ANALYSIS; MAST CELLS; IMAGE PROCESSING; CHARGE-COUPLED DEVICES; ELECTROPHORESIS
Citation Formats
Su, H, and Yeung, E S. Study of cell degranulation with simultaneous microscope imaging and capillary electrophoresis. United States: N. p., 1999.
Web. doi:10.1366/0003702991947522.
Su, H, & Yeung, E S. Study of cell degranulation with simultaneous microscope imaging and capillary electrophoresis. United States. https://doi.org/10.1366/0003702991947522
Su, H, and Yeung, E S. 1999.
"Study of cell degranulation with simultaneous microscope imaging and capillary electrophoresis". United States. https://doi.org/10.1366/0003702991947522.
@article{osti_686512,
title = {Study of cell degranulation with simultaneous microscope imaging and capillary electrophoresis},
author = {Su, H and Yeung, E S},
abstractNote = {The physical release of single granules from individual rat peritoneal mast cells (RPMCs) was monitored by video recording of the degranulating cells by a high-resolution charge-coupled device (CCD) microscope system. The bright granular core disappears from the image as the vesicular content is dissolved on contact with the extra-cellular fluid. After a fixed time delay, the exocytotic product, serotonin, was detected by capillary electrophoresis coupled with laser-induced native fluorescence (LINF-CE). The timing of the two events is mostly correlated, which supports a fast release mechanism of the granular products. {copyright} {ital 1999} {ital Society for Applied Spectroscopy}},
doi = {10.1366/0003702991947522},
url = {https://www.osti.gov/biblio/686512},
journal = {Applied Spectroscopy},
number = 7,
volume = 53,
place = {United States},
year = {Thu Jul 01 00:00:00 EDT 1999},
month = {Thu Jul 01 00:00:00 EDT 1999}
}
Other availability
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.