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Title: Ketone-body utilization by homogenates of adult rat brain

Abstract

The regulation of ketone-body metabolism and the quantitative importance of ketone bodies as lipid precursors in adult rat brain has been studied in vitro. Utilization of ketone bodies and of pyruvate by homogenates of adult rat brain was measured and the distribution of /sup 14/C from (3-/sup 14/C)ketone bodies among the metabolic products was analysed. The rate of ketone-body utilization was maximal in the presence of added Krebs-cycle intermediates and uncouplers of oxidative phosphorylation. The consumption of acetoacetate was faster than that of D-3-hydroxybutyrate, whereas, pyruvate produced twice as much acetyl-CoA as acetoacetate under optimal conditions. Millimolar concentrations of ATP in the presence of uncoupler lowered the consumption of ketone bodies but not of pyruvate. Indirect evidence is presented suggesting that ATP interferes specifically with the mitochondrial uptake of ketone bodies. Interconversion of ketone bodies and the accumulation of acid-soluble intermediates (mainly citrate and glutamate) accounted for the major part of ketone-body utilization, whereas only a small part was oxidized to CO/sub 2/. Ketone bodies were not incorporated into lipids or protein. We conclude that adult rat-brain homogenates use ketone bodies exclusively for oxidative purposes.

Authors:
;
Publication Date:
Research Org.:
Laboratory of Veterinary Biochemistry State University of Utrecht, Netherlands
OSTI Identifier:
6857564
Resource Type:
Journal Article
Journal Name:
Neurochem. Res.; (United States)
Additional Journal Information:
Journal Volume: 7:6
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BRAIN; KETONES; METABOLISM; ACETOACETATES; ATP; BUTYRIC ACID; CARBON 14 COMPOUNDS; CARBON DIOXIDE; COENZYMES; LIPIDS; MITOCHONDRIA; OXIDATION; PHOSPHORYLATION; PYRUVIC ACID; RATS; TRACER TECHNIQUES; ANIMALS; BODY; CARBON COMPOUNDS; CARBON OXIDES; CARBOXYLIC ACID SALTS; CARBOXYLIC ACIDS; CELL CONSTITUENTS; CENTRAL NERVOUS SYSTEM; CHALCOGENIDES; CHEMICAL REACTIONS; ISOTOPE APPLICATIONS; KETO ACIDS; LABELLED COMPOUNDS; MAMMALS; MONOCARBOXYLIC ACIDS; NERVOUS SYSTEM; NUCLEOTIDES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANOIDS; ORGANS; OXIDES; OXYGEN COMPOUNDS; RODENTS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Lopes-Cardozo, M, and Klein, W. Ketone-body utilization by homogenates of adult rat brain. United States: N. p., 1982. Web. doi:10.1007/BF00965522.
Lopes-Cardozo, M, & Klein, W. Ketone-body utilization by homogenates of adult rat brain. United States. https://doi.org/10.1007/BF00965522
Lopes-Cardozo, M, and Klein, W. 1982. "Ketone-body utilization by homogenates of adult rat brain". United States. https://doi.org/10.1007/BF00965522.
@article{osti_6857564,
title = {Ketone-body utilization by homogenates of adult rat brain},
author = {Lopes-Cardozo, M and Klein, W},
abstractNote = {The regulation of ketone-body metabolism and the quantitative importance of ketone bodies as lipid precursors in adult rat brain has been studied in vitro. Utilization of ketone bodies and of pyruvate by homogenates of adult rat brain was measured and the distribution of /sup 14/C from (3-/sup 14/C)ketone bodies among the metabolic products was analysed. The rate of ketone-body utilization was maximal in the presence of added Krebs-cycle intermediates and uncouplers of oxidative phosphorylation. The consumption of acetoacetate was faster than that of D-3-hydroxybutyrate, whereas, pyruvate produced twice as much acetyl-CoA as acetoacetate under optimal conditions. Millimolar concentrations of ATP in the presence of uncoupler lowered the consumption of ketone bodies but not of pyruvate. Indirect evidence is presented suggesting that ATP interferes specifically with the mitochondrial uptake of ketone bodies. Interconversion of ketone bodies and the accumulation of acid-soluble intermediates (mainly citrate and glutamate) accounted for the major part of ketone-body utilization, whereas only a small part was oxidized to CO/sub 2/. Ketone bodies were not incorporated into lipids or protein. We conclude that adult rat-brain homogenates use ketone bodies exclusively for oxidative purposes.},
doi = {10.1007/BF00965522},
url = {https://www.osti.gov/biblio/6857564}, journal = {Neurochem. Res.; (United States)},
number = ,
volume = 7:6,
place = {United States},
year = {Tue Jun 01 00:00:00 EDT 1982},
month = {Tue Jun 01 00:00:00 EDT 1982}
}