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Title: Therapeutic administration of recombinant human granulocyte colony-stimulating factor accelerates hemopoietic regeneration and enhances survival in a murine model of radiation-induced myelosuppression

The primary cause of death after radiation exposure is infection resulting from myelosuppression. Because granulocytes play a critical role in host defense against infection and because granulocyte proliferation and differentiation are enhanced by granulocyte colony-stimulating factor (G-CSF), this agent was evaluated for the ability to accelerate hemopoietic regeneration and to enhance survival in irradiated mice. C3H/HeN mice were irradiated and G-CSF (2.5 micrograms/day, s.c.) or saline was administered on days 3-12, 1-12 or 0-12 post-irradiation. Bone marrow, splenic and peripheral blood cellularity, and bone marrow and splenic granulocyte-macrophage progenitor cell recoveries were evaluated in mice exposed to 6.5 Gy. Mice exposed to 8 Gy were evaluated for multipotent hemopoietic stem cell recovery (using endogenous spleen colony-forming units) and enhanced survival. Results demonstrated that therapeutic G-CSF (1) accelerates hemopoietic regeneration after radiation-induced myelosuppression, (2) enhances survival after potentially lethal irradiation and (3) is most effective when initiated 1 h following exposure.
Authors:
; ; ;  [1]
  1. (Armed Forces Radiobiology Research Institute, Bethesda, MD (USA))
Publication Date:
OSTI Identifier:
6853390
Resource Type:
Journal Article
Resource Relation:
Journal Name: International Journal of Cell Cloning; (USA); Journal Volume: 8:2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; GROWTH FACTORS; RADIOSENSITIVITY EFFECTS; LEUKOCYTES; CELL PROLIFERATION; ANEMIAS; MICE; RADIATION INJURIES; SURVIVAL TIME; WHOLE-BODY IRRADIATION; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BIOLOGICAL RADIATION EFFECTS; BLOOD; BLOOD CELLS; BODY FLUIDS; DISEASES; EXTERNAL IRRADIATION; HEMIC DISEASES; INJURIES; IRRADIATION; MAMMALS; MATERIALS; MITOGENS; ORGANIC COMPOUNDS; PROTEINS; RADIATION EFFECTS; RODENTS; SYMPTOMS; VERTEBRATES 560152* -- Radiation Effects on Animals-- Animals