Therapeutic administration of recombinant human granulocyte colony-stimulating factor accelerates hemopoietic regeneration and enhances survival in a murine model of radiation-induced myelosuppression

Journal Article · Thu Mar 01 00:00:00 EST 1990 · International Journal of Cell Cloning; (USA)

DOI:https://doi.org/10.1002/stem.5530080204· OSTI ID:6853390

Patchen, M L; MacVittie, T J; Solberg, B D; Souza, L M ^[1]

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1. Armed Forces Radiobiology Research Institute, Bethesda, MD (USA)

The primary cause of death after radiation exposure is infection resulting from myelosuppression. Because granulocytes play a critical role in host defense against infection and because granulocyte proliferation and differentiation are enhanced by granulocyte colony-stimulating factor (G-CSF), this agent was evaluated for the ability to accelerate hemopoietic regeneration and to enhance survival in irradiated mice. C3H/HeN mice were irradiated and G-CSF (2.5 micrograms/day, s.c.) or saline was administered on days 3-12, 1-12 or 0-12 post-irradiation. Bone marrow, splenic and peripheral blood cellularity, and bone marrow and splenic granulocyte-macrophage progenitor cell recoveries were evaluated in mice exposed to 6.5 Gy. Mice exposed to 8 Gy were evaluated for multipotent hemopoietic stem cell recovery (using endogenous spleen colony-forming units) and enhanced survival. Results demonstrated that therapeutic G-CSF (1) accelerates hemopoietic regeneration after radiation-induced myelosuppression, (2) enhances survival after potentially lethal irradiation and (3) is most effective when initiated 1 h following exposure.

• OSTI ID: 6853390
• Journal Information: International Journal of Cell Cloning; (USA), Vol. 8:2; ISSN 0737-1454
• Country of Publication: United States
• Language: English

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560152* - Radiation Effects on Animals- Animals