Study of heat and radiation response of a malignant, melanin-producing cell line derived from C3H 10T1/2 cells transformed in culture by radiation

Journal Article · Mon Dec 01 00:00:00 EST 1986 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(86)90014-3· OSTI ID:6851372

Raaphorst, G P; Vadasz, J; Azzam, E I

The mouse C3H 10T1/2 cell line was transformed to the malignant state using ionizing radiation. One of the transformed lines (R25) that was isolated, displayed some properties similar to malignant melanoma cells. The cells became dark and pigmented after prolonged time in culture and this cell line produced tumors in C3H mice. The radiation survival curve of R25 had a large shoulder which was also observed for human melanoma cell lines. R25 was more resistant to heating at 45.0 degrees C than the normal cell line. Heating at 45.0 degrees C before irradiation resulted in a reduction of the survival curve shoulder. The heat and radiation sensitivity of R25 did not appear to be related to the melanin content of these cells.

• Research Organization: Medical Physics, Ottawa Regional Cancer Centre, Ontario
• OSTI ID: 6851372
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Vol. 12
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
RADIOSENSITIVITY
MELANOMAS
RADIOINDUCTION
CELL CULTURES
CELL TRANSFORMATIONS
HYPERTHERMIA
IONIZING RADIATIONS
MELANIN
MICE
SURVIVAL TIME
ANIMALS
BIOLOGICAL EFFECTS
BODY TEMPERATURE
DISEASES
HYDROXY COMPOUNDS
MAMMALS
NEOPLASMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture