Chromosomal localization, genomic structure, and allelic polymorphism of the human CD79a (lg-{alpha}/mb-1) gene
- North Shore University Hospital, Manhasset, NY (United States)
- Lawrence Livermore National Lab., CA (United States)
The germline DNA sequence of the human CD79a (Ig-{alpha}/mb-1) gene was determined by polymerase chain reaction sequencing of a cosmid clone derived from an arrayed human chromosome 19 library. The CD79a gene was localized to chromosome 19q13.2; this localization places the gene within the CEA-like gene cluster with the following gene order: -CEA-CGM1-CD79a-RPS11-ATP1A3-BGP-CGM9-. The genomic organization of the human CD79a gene resembles the mouse counterpart with five exons interrupted by four introns. Computer analyses suggest the presence of transcription regulatory elements known to be important in the regulation of mouse CD79a (AP-1, EBF, AP-2, MUF2, and SP-1 sites), as well as elements not found in the mouse gene (an NK-kB binding site and a series of E-box motifs). Similar to the mouse gene, the 5{prime} flanking region of human CD79a lacks a TATA box; however, unlike mouse CD79a, a classical octamer motif could not be identified in the human gene. Finally, a new Rsa I restriction fragment length polymorphism was defined in the non-coding regions of the human gene. 64 refs., 4 figs., 2 tabs.
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 68497
- Journal Information:
- Immunogenetics, Vol. 40, Issue 4; Other Information: PBD: 1994
- Country of Publication:
- United States
- Language:
- English
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