RNA polymerase III transcription in higher plants: (Annual) performance report, May 1, 1986 through December 20, 1986
Technical Report
·
OSTI ID:6847174
From wheat germ, we have isolated and partially purified the RNA polymerase III. We have shown it to be functional for tRNA synthesis in combination with yeast transcription factors tau (TFIIIC) and B. In addition, by the test of specific DNA binding, we have isolated a putative wheat TFIIIC. Several critical tests have been made, using human (HeLa cell) RNA polymerase III and its factors as a model system, to see whether complementation of an incomplete yeast Pol III system with factors of heterologous origin is likely to be successful. 5 refs.
- Research Organization:
- Washington Univ., Seattle (USA). Dept. of Genetics
- DOE Contract Number:
- FG06-86ER13517
- OSTI ID:
- 6847174
- Report Number(s):
- DOE/ER/13517-1; ON: DE87005548
- Country of Publication:
- United States
- Language:
- English
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