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Title: RNA polymerase III transcription in higher plants: (Annual) performance report, May 1, 1986 through December 20, 1986

From wheat germ, we have isolated and partially purified the RNA polymerase III. We have shown it to be functional for tRNA synthesis in combination with yeast transcription factors tau (TFIIIC) and B. In addition, by the test of specific DNA binding, we have isolated a putative wheat TFIIIC. Several critical tests have been made, using human (HeLa cell) RNA polymerase III and its factors as a model system, to see whether complementation of an incomplete yeast Pol III system with factors of heterologous origin is likely to be successful. 5 refs.
Authors:
Publication Date:
OSTI Identifier:
6847174
Report Number(s):
DOE/ER/13517-1
ON: DE87005548
DOE Contract Number:
FG06-86ER13517
Resource Type:
Technical Report
Research Org:
Washington Univ., Seattle (USA). Dept. of Genetics
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; NUCLEOPROTEINS; FRACTIONATION; RNA POLYMERASES; TRANSFER RNA; WHEAT; CEREALS; ENZYMES; GRASS; NUCLEIC ACIDS; NUCLEOTIDYLTRANSFERASES; ORGANIC COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; PLANTS; POLYMERASES; PROTEINS; RNA; SEPARATION PROCESSES; TRANSFERASES 550200* -- Biochemistry