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Title: Radioenzymatic assay for quinolinic acid

A new and rapid method for the determination of the excitotoxic tryptophan metabolite quinolinic acid is based on its enzymatic conversion to nicotinic acid mononucleotide and, in a second step utilizing (/sup 3/H)ATP, further to (/sup 3/H) deamido-NAD. Specificity of the assay is assured by using a highly purified preparation of the specific quinolinic acid-catabolizing enzyme, quinolinic acid phosphoribosyltransferase, in the initial step. The limit of sensitivity was found to be 2.5 pmol of quinolinic acid, sufficient to conveniently determine quinolinic acid levels in small volumes of human urine and blood plasma.
Authors:
; ; ;
Publication Date:
OSTI Identifier:
6846673
Resource Type:
Journal Article
Resource Relation:
Journal Name: Anal. Biochem.; (United States); Journal Volume: 1
Research Org:
Maryland Psychiatric Research Center, Baltimore
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; METABOLITES; RADIOENZYMATIC ASSAY; PHOSPHOTRANSFERASES; ENZYME ACTIVITY; ATP; LABELLED COMPOUNDS; NAD; PYRIDINES; TRACER TECHNIQUES; TRITIUM COMPOUNDS; TRYPTOPHAN; AMINO ACIDS; AROMATICS; AZAARENES; AZINES; AZOLES; CARBOXYLIC ACIDS; COENZYMES; ENZYMES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; INDOLES; ISOTOPE APPLICATIONS; NUCLEOTIDES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; PYRROLES; TRANSFERASES 550201* -- Biochemistry-- Tracer Techniques