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Title: Pertussis toxin treatment does not block inhibition by atrial natriuretic factor of aldosterone secretion in cultured bovine zona glomerulosa cells

The authors have previously reported that atrial natriuretic factor (ANF) potently inhibits PGE or forskolin-stimulation aldosterone secretion in bovine zona glomerulosa (ZG) by acting through specific high affinity receptors. In order to evaluate the functional role of the regulatory protein N/sub i/ and the inhibition of adenylate cyclase activity (AC) in ZG, the authors have studied the effect of treatment with PT on inhibition by ANF of aldosterone production. Primary cultures of ZG were treated for 18 hours in serum-free F12 medium with (0-100 ng/ml PT). No effect of PT pretreatment was observed either on basal, PGE-stimulated or ANF-inhibited levels of steroidogenesis. When membranes prepared from control ZG were ADP-ribosylated with (/sup 32/P) NAD in the presence of PT, two toxin-specific bands with 39 Kd and 41 Kd were documented on SDS gel. Cell pretreatment with as low as 1 ng/ml drastically reduced further labelling of these two bands while higher doses completely abolished them. Since PT treatment covalently modifies completely the toxin substrate without altering ANF inhibition of adrenal steroidogenesis, the authors conclude that N/sub i/ is not involved in the mode of action of ANF on aldosterone production.
Authors:
;
Publication Date:
OSTI Identifier:
6846332
Report Number(s):
CONF-8604222-
Journal ID: CODEN: FEPRA; TRN: 87-008625
Resource Type:
Journal Article
Resource Relation:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States); Journal Volume: 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
Research Org:
Institut de Recherches Cliniques de Montreal, Quebec
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ALDOSTERONE; SECRETION; CYCLASES; ENZYME ACTIVITY; PROTEINS; BIOLOGICAL EFFECTS; TOXINS; BIOCHEMICAL REACTION KINETICS; CATTLE; CELL CULTURES; CELL MEMBRANES; INHIBITION; NAD; PHOSPHORUS 32; TRACER TECHNIQUES; VALENCE; ADRENAL HORMONES; ALDEHYDES; ANIMALS; ANTIGENS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CELL CONSTITUENTS; COENZYMES; CORTICOSTEROIDS; DAYS LIVING RADIOISOTOPES; DOMESTIC ANIMALS; ENZYMES; HORMONES; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; ISOTOPES; KETONES; KINETICS; LIGHT NUCLEI; LYASES; MAMMALS; MATERIALS; MEMBRANES; MINERALOCORTICOIDS; NUCLEI; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; PREGNANES; RADIOISOTOPES; REACTION KINETICS; RUMINANTS; STEROID HORMONES; STEROIDS; TOXIC MATERIALS; VERTEBRATES 550201* -- Biochemistry-- Tracer Techniques