Culture and characteristics of hormone-responsive neuroblastoma x glioma hybrid cells

Journal Article · Tue Jan 01 00:00:00 EST 1985 · Methods Enzymol.; (United States)

DOI:https://doi.org/10.1016/0076-6879(85)09096-6· OSTI ID:6846118

Hamprecht, B; Glaser, T; Reiser, G; Bayer, E; Propst, F

Neuroblastoma x glioma hybrid cells were generated by cell fusion of the 6-thioguanine-resistant clonal mouse neuroblastoma cells and the bromodeoxyuridine-resistant rat glioma cells, selection, and cloning. Every characteristics generally ascribed to neurons has been observed with the hybrid cells. The paper explores the morphological differentiation of hybrid cells, procedures for testing the hormonal regulation of intracellular levels of cyclic, (/sup 3/H)AMP in hybrid cells, hormonal regulation of adenylate cyclase in homogenates of hyrbid cells, intracellular levels of cyclic GMP, and uptake of guanidinium ions in hybrid cells.

• OSTI ID: 6846118
• Journal Information: Methods Enzymol.; (United States), Vol. 109
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
NERVE CELLS
BIOCHEMICAL REACTION KINETICS
HYBRIDIZATION
TUMOR CELLS
AMP
CELL CULTURES
CLONE CELLS
CYCLASES
EXPERIMENTAL DATA
GLIOMAS
GUANIDINES
HORMONES
MICE
MORPHOLOGY
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
CARBONIC ACID DERIVATIVES
DATA
DISEASES
ENZYMES
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LYASES
MAMMALS
NEOPLASMS
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
REACTION KINETICS
RODENTS
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques