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Title: Activation of tumoricidal properties in human blood monocytes by muramyl dipeptide requires specific intracellular interaction

Abstract

The purpose of this study was to identify the mechanism by which muramyl dipeptide (MDP) activates antitumor cytotoxic properties in normal and interferon-..gamma.. (IFN-..gamma..)-primed human peripheral blood monocytes. The structurally and functionally active MDP analog, nor-muramyl dipeptide (nor-MDP), and (/sup 3/H)nor-MDP were used as reference glycopeptides. Direct activation of normal, noncytotoxic monocytes by nor-MDP was enhanced its encapsulation within multilamellar vesicles (MLV). Studies with (/sup 3/H)nor-MDP revealed that the activation of monocytes by nor-MDP was not attributable to its interaction with a specific cell surface receptor, nor did it result merely from the internalization by monocytes of glycopeptide. Subthreshold concentrations of nor-MDP could activate tumor cytotoxic properties in IFN-..gamma..-primed monocytes. The intracellular interaction of (/sup 3/H)nor-MDP with IFN-..gamma..-primed monocytes was specific in that intracellular levels of radiolabeled material could be displaced and recovered as intact molecules by unlabeled nor-MDP, but not by a biologically inactive MDP stereoisomer. Collectively, these results suggest that the activation of tumoricidal properties in human blood monocytes by MDP occurs subsequent to intracellular interaction with specific MDP receptors.

Authors:
;
Publication Date:
Research Org.:
Univ. of Texas System Cancer Center, Houston
OSTI Identifier:
6845961
Resource Type:
Journal Article
Journal Name:
J. Immunol.; (United States)
Additional Journal Information:
Journal Volume: 136:6
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANIMAL CELLS; CELL KILLING; BACTERIA; CELL WALL; PEPTIDES; INTERFERON; BIOLOGICAL EFFECTS; DYNAMIC FUNCTION STUDIES; LABELLED COMPOUNDS; MONOCYTES; TRITIUM; TUMOR CELLS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CELL CONSTITUENTS; GROWTH FACTORS; HYDROGEN ISOTOPES; ISOTOPES; LEUKOCYTES; LIGHT NUCLEI; LYMPHOKINES; MATERIALS; MICROORGANISMS; MITOGENS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PROTEINS; RADIOISOTOPES; YEARS LIVING RADIOISOTOPES; 550301* - Cytology- Tracer Techniques

Citation Formats

Fogler, W E, and Fidler, I J. Activation of tumoricidal properties in human blood monocytes by muramyl dipeptide requires specific intracellular interaction. United States: N. p., 1986. Web.
Fogler, W E, & Fidler, I J. Activation of tumoricidal properties in human blood monocytes by muramyl dipeptide requires specific intracellular interaction. United States.
Fogler, W E, and Fidler, I J. 1986. "Activation of tumoricidal properties in human blood monocytes by muramyl dipeptide requires specific intracellular interaction". United States.
@article{osti_6845961,
title = {Activation of tumoricidal properties in human blood monocytes by muramyl dipeptide requires specific intracellular interaction},
author = {Fogler, W E and Fidler, I J},
abstractNote = {The purpose of this study was to identify the mechanism by which muramyl dipeptide (MDP) activates antitumor cytotoxic properties in normal and interferon-..gamma.. (IFN-..gamma..)-primed human peripheral blood monocytes. The structurally and functionally active MDP analog, nor-muramyl dipeptide (nor-MDP), and (/sup 3/H)nor-MDP were used as reference glycopeptides. Direct activation of normal, noncytotoxic monocytes by nor-MDP was enhanced its encapsulation within multilamellar vesicles (MLV). Studies with (/sup 3/H)nor-MDP revealed that the activation of monocytes by nor-MDP was not attributable to its interaction with a specific cell surface receptor, nor did it result merely from the internalization by monocytes of glycopeptide. Subthreshold concentrations of nor-MDP could activate tumor cytotoxic properties in IFN-..gamma..-primed monocytes. The intracellular interaction of (/sup 3/H)nor-MDP with IFN-..gamma..-primed monocytes was specific in that intracellular levels of radiolabeled material could be displaced and recovered as intact molecules by unlabeled nor-MDP, but not by a biologically inactive MDP stereoisomer. Collectively, these results suggest that the activation of tumoricidal properties in human blood monocytes by MDP occurs subsequent to intracellular interaction with specific MDP receptors.},
doi = {},
url = {https://www.osti.gov/biblio/6845961}, journal = {J. Immunol.; (United States)},
number = ,
volume = 136:6,
place = {United States},
year = {Sat Mar 15 00:00:00 EST 1986},
month = {Sat Mar 15 00:00:00 EST 1986}
}