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Title: Splenic microenvironment and self recognition as factors in allograft rejection in rats. A study using indium-111-labeled cells

Abstract

Splenectomy facilitates organ allograft survival in some rat strains, and in weak donor-recipient histoincompatible pairs. We have found using a heart spleen twin graft model, using ACI rats as recipients and Lewis rats as donors, that the transplanted heart will survive in most recipients after delayed host splenectomy. The presence of a viable mass of splenic tissue will allow rejection to proceed only when the transplanted spleen is of host origin, and not when it comes from the donor (i.e., when it is allogeneic). The use of 111In-labeled cells has allowed us to show that lymphocyte traffic and trapping is markedly altered in the transplanted allogeneic spleens, when compared with control transplanted syngeneic spleens. Thus, despite the presence of the splenic ''microenvironment,'' cardiac allograft rejection does not occur in the absence of syngeneic splenic tissue. We conclude that the role of the spleen in the immune response is to facilitate the recognition of self and the acquisition of alloreactivity in weak responder rat strains and donor-recipient pairs.

Authors:
; ;
Publication Date:
Research Org.:
Univ. of Illinois College of Medicine at Chicago
OSTI Identifier:
6845086
Resource Type:
Journal Article
Journal Name:
Transplantation; (United States)
Additional Journal Information:
Journal Volume: 5
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; HEART; TRANSPLANTS; SPLEEN; BIOLOGICAL MODELS; GRAFT-HOST REACTION; INDIUM 111; RATS; SPLENECTOMY; SURVIVAL TIME; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; CARDIOVASCULAR SYSTEM; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; INDIUM ISOTOPES; INTERMEDIATE MASS NUCLEI; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MAMMALS; MEDICINE; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANS; RADIOISOTOPES; RODENTS; SURGERY; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Pollak, R, Blanchard, J M, and Lazda, V A. Splenic microenvironment and self recognition as factors in allograft rejection in rats. A study using indium-111-labeled cells. United States: N. p., 1986. Web.
Pollak, R, Blanchard, J M, & Lazda, V A. Splenic microenvironment and self recognition as factors in allograft rejection in rats. A study using indium-111-labeled cells. United States.
Pollak, R, Blanchard, J M, and Lazda, V A. 1986. "Splenic microenvironment and self recognition as factors in allograft rejection in rats. A study using indium-111-labeled cells". United States.
@article{osti_6845086,
title = {Splenic microenvironment and self recognition as factors in allograft rejection in rats. A study using indium-111-labeled cells},
author = {Pollak, R and Blanchard, J M and Lazda, V A},
abstractNote = {Splenectomy facilitates organ allograft survival in some rat strains, and in weak donor-recipient histoincompatible pairs. We have found using a heart spleen twin graft model, using ACI rats as recipients and Lewis rats as donors, that the transplanted heart will survive in most recipients after delayed host splenectomy. The presence of a viable mass of splenic tissue will allow rejection to proceed only when the transplanted spleen is of host origin, and not when it comes from the donor (i.e., when it is allogeneic). The use of 111In-labeled cells has allowed us to show that lymphocyte traffic and trapping is markedly altered in the transplanted allogeneic spleens, when compared with control transplanted syngeneic spleens. Thus, despite the presence of the splenic ''microenvironment,'' cardiac allograft rejection does not occur in the absence of syngeneic splenic tissue. We conclude that the role of the spleen in the immune response is to facilitate the recognition of self and the acquisition of alloreactivity in weak responder rat strains and donor-recipient pairs.},
doi = {},
url = {https://www.osti.gov/biblio/6845086}, journal = {Transplantation; (United States)},
number = ,
volume = 5,
place = {United States},
year = {Sat Nov 01 00:00:00 EST 1986},
month = {Sat Nov 01 00:00:00 EST 1986}
}